结直肠癌组织中HK2与AKT的表达、临床意义及其相关性分析

Expression,clinical significance and correlation analysis of HK2 and AKT in colorectal cancer tissues

目的:对比己糖激酶2(hexokinase 2,HK2)和丝氨酸/苏氨酸蛋白激酶(serin/threonine kinase,AKT)在结直肠癌组织及癌旁正常肠上皮组织中的表达,分析二者与结直肠癌相关临床病理特征的相关性及其预后价值,探索HK2与AKT表达间的相关性。方法:利用免疫组化法检测90例结直肠癌患者肿瘤组织中HK2及AKT的蛋白表达,以癌旁正常肠上皮组织作为对照。结合患者临床病理特征分析两蛋白的临床意义、预后价值及表达相关性。结果:90例结直肠癌组织中,HK2和AKT蛋白表达相较于癌旁正常肠上皮组织均呈更高表达。二者在结直肠癌组织中的表达与患者性别、年龄、肿瘤原发部位、肿瘤大体分型、肿瘤大小及T分期均无关,与淋巴结转移、远处转移和TNM分期均相关(P<0.05)。AKT表达还与肿瘤分化程度相关(P<0.05)。Kaplan-Meier生存分析显示:HK2、AKT与预后均显著相关,高表达患者的总生存时间均少于低表达患者(P<0.05)。COX分析显示:远处转移、HK2和AKT是影响结直肠癌患者预后的独立因素(P<0.05)。相关性分析提示:在结直肠癌组织中,HK2和AKT的蛋白表达存在显著正相关性(r=0.579,P<0.001)。结论:HK2与AKT蛋白均为结直肠癌危险因素,二者表达正相关。

Objective:To compare the expression of hexokinase 2(HK2)and the serin/threonine kinase(AKT)in colorectal cancer and para-carcinoma tissue,to analyze the correlation between their expressions and the pathological parameters of colorectal cancer,to analyze the prognostic value of HK2 and AKT,and explore the correlation between them.Methods:The protein level of HK2 and AKT was detected by immunohistochemical method in 90 specimens of colorectal cancer and 90 of para-carcinoma tissue.The clinical significance,prognostic value and the correlation between the two proteins were analyzed according to all clinicopathological parameters.Results:The expression of HK2 and AKT in 90 colorectal cancer specimens were higher than those of para-carcinoma tissue.Their expressions in colorectal cancer were not correlated with patients'gender,age,primary site,gross type,tumor size and T stage,but related with lymph node metastasis,distant metastasis and TNM stage(P<0.05).AKT expression was also correlated with tumor differentiation(P<0.05).Kaplan-Meier survival analysis showed that HK2 and AKT expression were significantly associated with prognosis.The overall survival time of patients with high HK2 or AKT expression was shorter than that of patients with low HK2 or AKT expression(P<0.05).COX analysis showed that distant metastasis,HK2 and AKT were independent prognostic factors in patients with colorectal cancer(P<0.05).Correlation analysis indicated that there was a significant positive correlation between HK2 and AKT protein expression in colorectal cancer tissues(r=0.579,P<0.001).Conclusion:HK2 and AKT are both risk factors in colorectal cancer,and their expressions are positively correlated.