血小板浓缩生长因子对正畸大鼠牙移动及牙周组织CCR-1和HO-1表达的影响

Effect of Concentrated Growth Factors on Movement of Orthodontic Tooth and Expressions of CCR-1 and HO-1 in Periodontium

目的:研究血小板浓缩生长因子(concentrated growth factors, CGF)对正畸大鼠牙移动及牙周组织CC类趋化因子受体-1(CC chemokine receptor-1, CCR-1)及血红素氧合酶-1(heme oxygenase-1, HO-1)表达的影响。方法:150只大鼠随机分为对照组和观察组,每组75只,两组均建立正畸牙移动模型,观察组每日腹腔注射10%CGF生理盐水溶液,对照组注射等体积生理盐水溶液,共注射14 d。分别检测第0 d、1 d、3 d、7 d和14 d两组大鼠的牙移动距离,TRAP染色并进行破骨细胞计数,检测牙周组织中CCR1和HO-1 mRNA的相对表达。结果:随正畸力作用时间的延长,两组大鼠牙移动距离均显著增长、破骨细胞计数显著增多、牙周组织中CCR1和HO-1 mRNA的相对表达均显著升高(P<0.05);对照组与观察组在第1 d和第3 d时牙移动距离相比无统计学差异(P>0.05),而在第7 d开始,观察组牙移动距离显著长于对照组(P<0.05);观察组大鼠破骨细胞计数和牙周组织中CCR1和HO-1 mRNA的相对表达均显著高于同时间点的对照组(P<0.05)。结论:CGF可促进正畸大鼠牙移动,增加破骨细胞数量,从而促进正畸牙移动和牙周组织重建,其机制可能与增加牙周组织中CCR1和HO-1的表达水平有关。

Objective: To investigate the effect of concentrated growth factors(CGF) on movement of orthodontic tooth and expressions of CC chemokine receptor-1(CCR-1) and heme oxygenase-1(HO-1) in periodontium. Methods: 150 rats were randomly divided into control group and observation group, with 75 rats in each group, orthodontic tooth model was established, the observation group was intraperitoneal injected of 10 % CGF normal saline solution, and the control group was intraperitoneal injected of equivalent volume of normal saline solution, 14 days of injection. On the 0th, 1st, 3rd, 7th and 14th days, the tooth movement distance of the two groups of rats was detected, TRAP staining and osteoclast count were performed to detect the relative expressions of CCR1 and HO-1 mRNA in periodontal tissue. Results: With the extension of orthodontic time, the tooth movement distances, osteoclast counts and relative expressions of CCR1 and HO-1 mRNA in periodontium of two groups were improved(P<0.05);the tooth movement distances of two groups at 1 d and 3 d had no significant difference(P>0.05), and the observation group was longer than control group from 7 d(P<0.05);the osteoclast counts and relative expressions of CCR1 and HO-1 mRNA in periodontium of observation group were higher than control group at the same time point(P<0.05). Conclusion: CGF can promote tooth movement in orthodontic rats and increase the number of osteoclasts,thereby promoting orthodontic tooth movement and periodontal tissue reconstruction. The mechanism may be related to the increase in the expression levels of CCR1 and HO-1 in periodontal tissue.