敲减miR-155对PCOS小鼠卵巢形态学及生殖内分泌的影响

Effect of miR-155 knockdown gene on ovarian morphology and reproductive endocrine in PCOS mice

[目的]研究miR-155敲减基因对多囊卵巢综合征(PCOS)小鼠卵巢形态及生殖内分泌的影响。[方法]取20只小鼠,采用CMC-来曲唑悬浮液灌胃建立PCOS模型,造模成功后将其随机分为miR-155基因敲减组与阴性对照组,各10只。检测两组小鼠血脂及血清性激素变化、卵巢形态、卵巢组织中GLUT4蛋白表达水平。[结果]miR-155基因敲减组中无优势卵泡,有较多小卵泡,无黄体,颗粒细胞减少,卵泡膜明显增厚,间质细胞增生显著;miR-155基因敲减组除FSH水平低于阴性对照组;其余血脂及血清性激素均高于阴性对照组,差异有统计学意义(P<0.05);miR-155基因敲减组中GLUT4蛋白低于阴性对照组,差异有统计学意义(P<0.05)。[结论]miR-155基因敲减可通过降低GLUT4蛋白分泌影响PCOS小鼠卵巢形态、内分泌及胰岛素抵抗功能。

[Objective]To study the effect of microRNA-155 knockdown gene on ovarian morphology and reproductive endocrine in mice with polycystic ovary syndrome(PCOS).[Method]Twenty mice were randomly divided into miR-155 gene knockdown group and negative control group,with 10 mice in each group.The changes of blood lipid,serum sex hormone,ovarian morphology and the expression of GLUT4 protein in ovarian tissue were detected.[Result]In miR-155 gene knockdown group,there were no dominant follicles,more small follicles,no corpus luteum,fewer granulosa cells,obvious thickening of follicular membrane and significant proliferation of stromal cells;The level of FSH in miR-155 gene knockout group was lower than that in negative control group;Other blood lipids and serum sex hormones were significantly higher than those in the negative control group(P<0.05);GLUT4 protein in miR-155 gene knockdown group was lower than that in negative control group,the difference was statistically significant(P<0.05).[Conclusion]miR-155 gene knockdown can affect the ovarian morphology,endocrine and insulin resistance of PCOS mice by reducing the secretion of GLUT4 protein.