苦参碱对急性淋巴细胞白血病小鼠肿瘤发展的影响

Effects of matrine on tumor development in mice with acute lymphoblastic leukemia

[目的]分析苦参碱对急性淋巴细胞白血病的影响及作用机制。[方法]30只NOD-SCD小鼠分为对照组、模型组、苦参碱组;模型组和苦参碱组小鼠建立白血病模型,苦参碱组小鼠用苦参碱灌胃治疗,对照组和模型组用等体积的生理盐水灌胃;记录各组小鼠体质量、小鼠存活率、外周血血常规;采用免疫组织化学法和Western Blot法检测组织中Caspase-3、VEGF、PI3K、Akt、p-PI3K、p-Akt、Bcl-2、Bax、β-actin蛋白表达水平。[结果]苦参碱组小鼠体质量、中性粒细胞、生存率、骨髓中Caspase-3阳性表达率、Bax表达水平明显高于模型组;苦参碱组白细胞、淋巴细胞、VEGF阳性表达率、p-PI3K/PI3K、p-AKT/AKT、Bcl-2表达水平明显低于模型组,且差异具统计学意义(P<0.05)。[结论]和模型组比较,苦参碱组PI3K/Akt水平显著降低,表明苦参碱能够通过PI3K/Akt通路抑制急性淋巴细胞白血病小鼠肿瘤发展。

[Objective]To analyze the effect and mechanism of matrine on acute lymphoblastic leukemia.[Method]Thirty 6-week-old SPF female NOD-SCD mice were selected and divided into control group,model group and matrine group;mice in model group and matrine group were used to establish leukemia model,and mice in matrine group were treated with matrine was administered by gavage,and the control group and model group were given an equal volume of normal saline;the body weight,mouse survival rate,and peripheral blood routine of each group were recorded;the bone marrow was detected by immunohistochemistry and Western Blot.The expression levels of Caspase-3 and VEGF in the tissue were detected by Western Blot method.The protein expression levels of PI3K,Akt,p-PI3K,p-Akt,Bcl-2,Bax andβ-actin in the mouse tissue were detected by Western Blot.[Result]The weight of mice in matrine group,neutrophils,survival rate,positive expression rate of Caspase-3 in bone marrow and Bax expression levels were significantly higher than those in the model group;white blood cells in the matrine group,lymphocytes,positive expression rate of VEGF,p-PI3K/PI3K,p-AKT/AKT,Bcl-2 expression level was significantly lower than that in the model group,and the difference was statistically significant(P<0.05).[Conclusion]Compared with the model group,the matrine group can be significantly reduced based on the level of PI3K/Akt,indicating that matrine can pass the PI3K/Akt pathway tumor development in mice with acute lymphoblastic leukemia.