基于免疫组化和癌症基因组图谱数据分析的脾酪氨酸激酶在宫颈癌中的表达研究

Expression of Syk Proteins in Carcinoma of the Cervix Based on Immunohistochemistry and Data Analysis from the Cancer Genome Atlas

目的:探讨Syk在宫颈癌中的表达及其临床意义。方法:应用免疫组化检测Syk在宫颈癌、癌前病变(CIN)和相应的正常宫颈组织中的表达。借助R2生物信息平台挖掘Syk在TCGA数据库305例宫颈鳞癌中的m RNA表达及其与预后的关系。结果:免疫组化结果显示,Syk在宫颈癌巢分化较好的中心区表达较强,在分化较低的癌巢周边区表达较弱。Syk染色主要定位在宫颈癌和正常宫颈组织的细胞质和细胞膜,正常宫颈组织基底细胞无Syk表达,8例CIN组织细胞核中可见Syk表达,但宫颈癌组织细胞核中未见Syk表达。Syk在宫颈癌、CIN和正常宫颈组织中的阳性率分别是76%、54%、40%,三组间的表达差异具有统计学意义(P=0.001)。Syk在深度浸润和淋巴结转移中表达较强。数据挖掘结果显示,Syk m RNA在305例不同临床分期的宫颈癌中均表达,Syk m RNA高表达组219例,Syk m RNA低表达组73例,其中13例生存数据缺失,Syk高表达组的患者预后较差。结论:Syk在宫颈癌中的表达提示Syk在宫颈癌中具有致癌蛋白的作用,Syk在某些CIN中的核表达可能与更好的预后相关。

Objective: Spleen tyrosine kinase(Syk), a non-receptor cytoplasmic tyrosine enzyme, is widely expressed in hematopoietic cells and epithelial ones. In the current study, expression and significance of Syk in cervical cancer was probed. Methods:Syk expression in cervical cancer, precancerous lesions(CIN) and corresponding normal cervical tissues was detected by immunohistochemistry. Data mining of Syk expression and its relationship with prognosis was performed in 305 tissue samples of cervical squamous carcinoma in the TCGA database resort to R2 bioinformatics platform. Results: Immunohistochemical results showed that Syk showed stronger staining in the highly differentiated central area than in poorly differentiated periphery of cancer nests. Syk staining was mainly located in the cytoplasm and cell membrane of cervical cancer and normal cervical tissue. There was no Syk expression in basal cells of normal cervical tissue. Syk expression was seen in the nuclei of 8 cases of CIN, but there was no Syk expression in the nuclei of cervical cancer. The positive rates of Syk in cervical cancer, CIN and normal cervical tissues were 76%, 54%and 40%, respectively. The expression difference among the three groups was statistically significant(P=0.001). Syk expression was higher in deeper invasion, and in lymph node metastasis group. Result of data mining showed that Syk m RNA expressed at different clinical stage of cervical cancer, Syk m RNA was high expression in 219 cases, low expression in 73 cases, and missing survival data in13 cases, the patients in Syk high expression had worse prognosis. Conclusions: The expression of Syk in cervical cancer suggests that Syk plays the role of carcinogenic protein in cervical cancer. The nuclear expression of Syk in some CIN may be related to better prognosis.