YKL-40、CCL27、CXCL10、Th17细胞及其相关细胞因子IL-17与皮肌炎患者疾病活动度和临床指标的相关性分析

Correlation Analysis of YKL-40,CCL27,CXCL10,Th17 Cells and its Related Cytokines IL-17 with Disease Activity and Clinical Indexes in Patients with Dermatomyositis

目的:探讨人软骨糖蛋白-39(YKL-40)、趋化因子配体-27(CCL27)、趋化因子配体-10(CXCL10)、辅助性T细胞17(Th17)及其相关细胞因子白细胞介素-17(IL-17)与皮肌炎患者疾病活动度和临床指标的相关性。方法:选择2020年2月至2022年2月河北北方学院附属第一医院皮肤科收治的80例皮肌炎患者为皮肌炎组,另选取同期40名健康体检人员作为对照组,检测对比两组血清YKL-40、CCL27、CXCL10、IL-17水平及外周血Th17细胞比例。皮肌炎患者根据病情分为活动期组和缓解期组,比较两组YKL-40、CCL27、CXCL10、IL-17水平、Th17细胞比例、皮肌炎相关临床指标及肌炎疾病活动性评估视觉模拟量表(MYOACT)评分,采用Pearson相关系数分析YKL-40、CCL27、CXCL10、IL-17水平、Th17细胞比例与MYOACT评分、皮肌炎相关临床指标的相关性。结果:皮肌炎组的YKL-40、CCL27、CXCL10、IL-17水平及Th17细胞比例均高于对照组(P<0.05)。活动期组的YKL-40、CCL27、CXCL10、IL-17水平及Th17细胞比例均高于缓解期组(P<0.05)。活动期组的MYOACT评分及红细胞沉降率(ESR)、铁蛋白(Fer)、乳酸脱氢酶(LDH)、肌酸激酶(CK)水平均高于缓解期组(P<0.05)。Pearson相关性分析显示,皮肌炎患者的YKL-40、CCL27、CXCL10、IL-17水平、Th17细胞比例与MYOACT评分、ESR、Fer、LDH、CK水平均呈正相关(P<0.05)。结论:血清YKL-40、CCL27、CXCL10、IL-17水平及外周血Th17细胞比例与皮肌炎病情严重程度有一定相关性,可作为皮肌炎病情的辅助评估指标。

Objective:To investigate the correlation of human cartilage glycoprotein-39(YKL)-40,chemokine CCL27(CCL27),chemokine CXCL10(CXCL10),helper T cell(Th)17 and its related cytokines interleukin(IL)-17 with disease activity and clinical indexes in patients with dermatomyositis.Methods:80 patients with dermatomyositis who were treated in the dermatology department of the First Affiliated Hospital of Hebei North University from February 2020 to February 2022 were selected as the dermatomyositis group,and 40 healthy physical examination personnel in the same period were selected as the control group.The levels of serum YKL-40,CCL27,CXCL10,IL-17 and peripheral blood Th17 cells ratio were detected and compared between the two groups.Patients with dermatomyositis were divided into active phase group and remission phase group according to their condition.The levels of YKL-40,CCL27,CXCL10,IL-17,Th17 cell ratio,dermatomyositis related clinical indexes and myositis disease activity assessment visual analogue scale(MYOACT)score were compared between the two groups.Pearson correlation coefficient was used to analyze the correlation between YKL-40,CCL27,CXCL10,IL-17,Th17 cell ratio and MYOACT score and dermatomyositis related clinical indexes.Results:The levels of YKL-40,CCL27,CXCL10,IL-17 and Th17 cell ratio in dermatomyositis group were higher than those in control group(P<0.05).The levels of YKL-40,CCL27,CXCL10,IL-17 and Th17 cell ratio in the active phase group were higher than those in the remission phase group(P<0.05).The MYOACT score,erythrocyte sedimentation rate(ESR),ferritin(Fer),lactate dehydrogenase(LDH)and creatine kinase(CK)levels in the active phase group were higher than those in the remission phase group(P<0.05).Pearson correlation analysis showed that the levels of YKL-40,CCL27,CXCL10,IL-17 and Th17 cells ratio in patients with dermatomyositis were positively correlated with MYOACT score,ESR,fer,LDH and CK(P<0.05).Conclusion:The levels of serum YKL-40,CCL27,CXCL10,IL-17 and peripheral blood Th17 cells ratio have a certain correlation with the severity of dermatomyositis condition,which can be used as an auxiliary evaluation index of dermatomyositis.