高效液相色谱-串联质谱法测定水中的六溴环十二烷和四溴双酚A

Determination of hexabromocyclododecanes and tetrabromobisphenol a in water by high performance liquid chromatography-tandem mass spectrometry

本文建立了同时测定水中六溴环十二烷(HBCDs)和四溴双酚A(TBBPA)的高效液相色谱-串联质谱方法.酸化后的水样(pH=2—4)经二氯甲烷液液萃取,硅胶固相萃取小柱净化后,经LC-MS/MS检测,采用同位素稀释法进行定量.目标化合物的方法检出限在0.3—0.5 ng·L^(−1)之间,不同水质样品平行添加的回收率为91.8%—117%,相对标准偏差是3.1%—11.9%.该方法灵敏度高、可靠性强,能够适用于环境各类水体中痕量HBCDs和TBBPA的监测要求.

A method for simultaneous determination of hexabromocyclododecanes(HBCDs)and tetrabromobisphenol A(TBBPA)in waters was developed using LC-MS/MS.After being adjusted to pH=2—4,the water sample was subjected to liquid–liquid extraction using dichloromethane,and the extractant was then cleaned up by silica gel solid phase extraction(SPE)cartridge.The resultant sample was analyzed using LC-MS/MS and quantified by isotope dilution method.The limits of detection were in the range of 0.3—0.5 ng·L^(−1),and spiking recoveries ranged from 91.8%to 117%,with relative standard deviations of 3.1%—11.9%.The established method was reliable,sensitive and was suitable for the determination of HBCDs and TBBPA in various types of water matrixes.