基于含量测定和指纹图谱的远志药效物质累积规律研究

Study on the accumulation law of effective substances of Polygalae Radix based on content determination and fingerprint

收集不同产地、不同生长年份的远志12批,对其水分、醇浸出物含量进行测定;基于HPLC测定远志■酮Ⅲ(polygalaxanthoneⅢ,PXⅢ)、3,6′-二芥子酰基蔗糖(3,6′-disinapoylsucrose, DISS)和细叶远志皂苷(tenuifolin, TEN)含量并建立指纹图谱,研究其药效物质累积规律。结果表明:随着生长年份的增加,远志根长度和直径增加,直径增加趋势更加显著;不同产地远志水分含量有明显的区别,汾阳产远志药材水分含量约4.0%,新绛产水分含量高于汾阳产约8.2%,12批药材水分检查均低于12%;浸出物含量介于38%~48%;PXⅢ含量介于0.11%~0.18%,DISS的含量介于0.50%~0.94%,TEN的含量介于1.37%~2.40%;PXⅢ、DISS累积规律较为相似;TEN在第二年和第四年的含量接近;新绛产药材指标成分总含量积累明显,在生长3年出现最大值;12批样品指纹图谱相似度介于0.823~0.962,共有35个共有峰,指认出2个共有峰,汾阳产样品指纹图谱相似度与新绛产没有明显差异,春采相似度高于秋采。本研究结果为远志栽培种植、采收时间提供参考;三种有效成分的含量测定结合指纹图谱可为远志药材的质量控制和评价提供参考。

To study the accumulation law of pharmacodynamic substances,12 batches of Polygalae Radix from different producing areas and different growth years were collected,and the contents of water and extract were determined.The contents of polygalaxanthoneⅢ(PXⅢ),3,6′-disinapoylsucrose(DISS),and tenuifolin(TEN)were determined by HPLC,and the fingerprint was established.The results showed that the root length and diameter of Polygalae Radix increased with the increase of the growth year,and the increasing trend of diameter was more significant.There are obvious differences in the water content of Polygalae Radix from different producing areas.The water content of Polygalae Radix from Fenyang is about 4.0%,that of Xinjiang is about 8.2%,which is higher than that from Fenyang,and that of 12 batches of Polygalae Radix is less than 12%.The content of extract is between 38%-48%.The content of PXⅢwas 0.11%-0.18%,and the content of DISS was 0.50%-0.94%,The content of TEN ranged from 1.37%-2.40%.The accumulation law of PXⅢand DISS was similar.The content of TEN in the second and fourth years was close;The total content of index components of Polygalae Radix produced in Xinjiang accumulated significantly,and the maximum value appeared in 3 years of growth.The fingerprint similarity of 12 batches of samples ranged from 0.823-0.962,with a total of 35 common peaks,and 2 of them were identified.There was no significant difference between the fingerprint similarity of Fenyang and Xinjiang,and the similarity of spring harvest was higher than that of the autumn harvest.In brief,the results of this study provide a reference for the cultivation and harvest time of Polygala tenuifolia.The content determination of three active components combined with fingerprint could provide a reference for the quality control and evaluation of Polygalae Radix.