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不同灰树花多糖提取及其体外抗氧化活性研究 被引量:2

Study on Extraction and Antioxidant Activity of Different Grifola frondosa Polysaccharides
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摘要 以4种不同产地和品种的灰树花为研究对象制备多糖,主要从得率、抗氧化性这2个方面比较灰树花品种间的差异。结果表明,4种灰树花(分别编号为D_(1)、D_(2)、X_(1)和X_(2))中D_(1)灰树花的多糖得率比较理想,并且总糖含量高、蛋白洗脱最为彻底。在抗氧化性方面,通过测定DPPH、ABTS及羟自由基清除率并比较IC_(50)值,结果表明,D_(2)、X_(1)品种灰树花多糖的抗氧化能力均最为优秀,其中DPPH自由基清除能力最强的是X_(1)多糖,其IC_(50)值为0.390±0.010 mg/mL,X_(2)多糖对ABTS自由基有较好的抑制能力,IC_(50)为0.160±0.004 mg/mL。此外,D_(2)多糖对羟自由基抑制能力的IC_(50)为0.650±0.051 mg/mL,是4种多糖中抑制活性最强的。综上所述,D_(1)品种的提取率较高,而D_(2)和X_(1)品种的抗氧化活性较好。试验可为灰树花作为食用真菌多糖产品开发原料提供一定的理论依据。 Four kinds of Grifola frondosa from different producing areas were used to prepare polysaccharides for the research.The differences among Grifola frondosa varieties were compared mainly from the following two aspects of yield and antioxidant capacity.The results showed that among the four kinds of Grifola frondosa(marked D_(1),D_(2),X_(1)and X_(2),respectively),the polysaccharide yield of D_(1)was ideal,with higher total sugar content and the most thorough protein elution.In terms of antioxidant properties,measure the scavenging rate of DPPH,ABTS and hydroxyl radicals,and compare IC_(50) values.It was concluded that D_(2)and X_(1)varieties of Grifola frondosa polysaccharides had the best antioxidant capacity.X_(1)polysaccharide showed the strongest DPPH free radicals scavenging ability,the IC_(50) of which was 0.390±0.010 mg/mL,and X_(2)polysaccharide presented excellent inhibitory ability on ABTS free radicals,with IC_(50) of 0.160±0.004 mg/mL.In addition,the IC_(50) of D_(2)polysaccharide to inhibit hydroxyl radicals was 0.650±0.051 mg/mL,which was the highest inhibition rate in the same group.The D_(1)variety had a higher extraction rate,while the D_(2)and X_(1)varieties had better antioxidant activities.This experiment could provide a certain theoretical basis for the development of edible fungal polysaccharide products.
作者 肖建勇 陈智超 朱育娴 陈可文 黄梓芮 刘斌 XIAO Jianyong;CHEN Zhichao;ZHU Yuxian;CHEN Kewen;HUANG Zirui;LIU Bin(College of Food Science,Fujian Agriculture and Forestry University,Fuzhou 350002;China National Engineering Research Center of JUNCAO Technology,Fujian Agriculture and Forestry University,Fuzhou 350002;College of Food Science and Engineering,Southwest Minzu University,Chengdu 610041)
出处 《食品工业》 CAS 2022年第8期59-63,共5页 The Food Industry
基金 福建省科技重大专项(2021NZ0101) 学科交叉融合推动菌草科学及产业高质量发展项目(XKJC-712021030)。
关键词 灰树花 多糖 得率 抗氧化 Grifola frondose polysaccharide yield antioxidant activity
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