生长调节剂对球孢白僵菌产孢和分生孢子性能的影响

Influence of Plant Growth Regulators on Sporulation and Conidia Characteristics of the Entomopathogenic Fungus Beauveria bassiana

球孢白僵菌在农林害虫生物防治中发挥着重要的作用,发酵周期和产孢量是其发酵工艺中的主要评价指标。本试验研究了生长调节剂对球孢白僵菌JG-17菌株产孢的促进作用。结果表明,通过外源法在菌落表层施用微量乙烯利,可以明显提高菌株JG-17的产孢量,以对8日菌龄表层施用100 ng/cm^(2)乙烯利处理最佳,产孢量为未处理对照的6.28倍。经乙烯利处理后形成的分生孢子,其耐热性、抗紫外线能力和对靶标害虫的杀虫活性与未处理对照没有显著性差异。在白僵菌培养过程中科学施用乙烯利,可有效提高白僵菌的产孢效率,研究为真菌生物农药工业化生产的效率提升提供了新的思路。

Entomopathogenic fungus Beauveria bassiana plays an important role in biological control of agricultural and forest pests. Fermentation period and sporulation quantity were regarded as the main evaluation indexes of B. bassiana fermentation. Influence of plant growth regulators on the conidial production of B. bassiana strain JG-17 was investigated. The results showed that the conidial production was significantly increased by spraying ethephon solution on the fungal colony at the vegetative growth stage. The sporulation in the fungal colony treated with 100 ng/cm^(2) of ethephon on the 8day of cultivation was 6.28 times of that in the control. No significant differences were observed in heat resistance, UV resistance and virulence against target pests of the conidia between the ethephon treatment and the control. A reasonable application of ethephon to the culture of B. bassiana can effectively improve the sporulation efficiency of B. bassiana. The results provide a new idea to improve mass production efficiency of fungal pesticides.