热转化西洋参茎叶皂苷的抗氧化及美白活性

Antioxidant and Whitening Activities of Heat-transformed American Ginseng Stem-leaf Saponins

该文研究了热转化前后西洋参茎叶皂苷中低极性人参皂苷含量的变化,并对转化前后的西洋参茎叶皂苷的抗氧化活性和美白活性进行了研究。结果表明,热转化过程使得西洋参茎叶皂苷中低极性人参皂苷含量由0.07 mg/mg增加到0.66 mg/mg。并且,在0.1~5 mg/mL浓度范围内,转化前后西洋参茎叶皂苷对DPPH自由基的最大清除率分别为16.65%和38.28%,对ABTS自由基的最大清除率分别为8.20%和41.94%,还原能力分别为3.24 mg TEAC/g DW和25.22 mg TEAC/g DW。此外,在0.025~0.1 mg/mL浓度范围内,热转化前后西洋参茎叶皂苷对B16细胞内酪氨酸酶活性最大抑制率分别为30.22%和33.54%,对B16细胞内黑色素生成的最大抑制率分别为17.27%和29.33%。综上,热转化过程提高了西洋参茎叶皂苷中的低极性人参皂苷含量,增强了西洋参茎叶皂苷的抗氧化活性和美白活性,在美容与医药领域具有很大的应用价值。

The levels of less-polar ginsenosides in American ginseng stem-leaf saponins and the antioxidant and whitening activities of American ginseng stem-leaf saponins before and after heat transformation were studied.The results showed that the content of less-polar ginsenosides in American ginseng stem-leaf saponins increased from 0.07 mg/mg to 0.66 mg/mg during heat transformation.Moreover,when the concentration of American ginseng stem-leaf saponins before and after transformation was in the range of 0.1~5 mg/mL,the maximum of DPPH free radical scavenging rates were 16.65%and 38.28%,respectively,ABTS free radical scavenging rates were 8.20%and 41.94%,respectively,and the reducing capacity was 3.24 mg TEAC/g DW and 25.22 mg TEAC/g DW,respectively.In addition,the concentration of American ginseng stem-leaf saponins before and after thermal transformation was in the range of 0.025~0.1 mg/mL,the maximum inhibition rates of tyrosinase activity in B16 cells were 30.22%and 33.54%,respectively,and the maximum inhibition rates of melanin production in B16 cells were 17.27%and 29.33%,respectively.In conclusion,the content of less-polar ginsenosides in American ginseng stem-leaf saponins was increased,and the antioxidant and whitening activities of American ginseng stem-leaf saponins were enhanced after heat transformation treatment,which has great application value in the fields of beauty and medicine.