一种席夫碱Al^(3+)荧光探针的制备及细胞成像应用

A Schiff Base Fluorescent Probe forAl^(3+):Synthesis and Application in Living Cells Imaging

以4⁃(二乙氨基)水杨醛与奥肼缩合制备了一例席夫碱类荧光探针1,通过1H NMR、^(13)C NMR和电喷雾电离质谱表征了1的结构。光谱分析实验结果显示,探针1可选择性与Al^(3+)作用,在495 nm处荧光发射峰显著增强。探针对Al^(3+)的检测灵敏度高,检测限低至1.44μmol·L^(-1)。结合理论计算,证实探针以三齿配位的模式,与Al^(3+)形成1∶1型稳定配合物。此外,该探针还可用于活细胞中Al^(3+)的检测。

A fluorescent probe 1 has been synthesized by the Schiff base condensation of 4⁃(diethylamino)salicylal⁃dehyde with oxamic hydrazide,which has been carefully characterized through 1H NMR,^(13)C NMR,and electrospray ionization mass spectrometer.Probe 1 exhibited a quite weak emission at 495 nm,which could selectively and sensi⁃tively recognize Al^(3+)from other cations by a notable fluorescence enhancement.The limit of detection of probe 1 for Al^(3+)was as low as 1.44μmol·L^(-1),indicating its high sensitivity.Based on theory calculation results,probe 1 is pro⁃posed to coordinate with Al with the ratio of 1∶1 in a tridentate mode.Moreover,the probe was applied to imaging Al^(3+)in living cells.