烷氧基三联吡啶钌配合物的合成、结构及其与DNA相互作用

Synthesis,Structure,and Interaction with DNA of Alkoxy Tribipyridine Ruthenium Complexes

制备了2种含长基链的烷氧基三联吡啶和2,2'⁃联吡啶配位的钌配合物(Ru⁃Cl、Ru⁃NCMe),通过质谱、核磁和单晶X射线衍射分析等进行了表征。从它们的晶体结构可以发现Ru⁃Cl配合物中第6个配位点为Cl-离子,在Ru⁃NCMe配合物中的第6个配位点为乙腈分子中的N原子。通过紫外可见吸收光谱和荧光光谱研究了这2种配合物与多种氨基酸和DNA的作用,发现这种长烷基链和刚性配位平面的配合物与DNA具有明显的特异性识别效应,进一步研究发现它们与DNA以插入方式和静电作用结合,实验与理论模拟结果一致。

Two ruthenium complexes(Ru⁃Cl,Ru⁃NCMe)containing long alkoxy tribipyridine and 2,2'⁃bipyridine were synthesized and characterized by mass spectrometry,NMR,single crystal X⁃ray diffraction analysis,etc.Based on their crystal structural information,it can be found that the sixth coordination position in complex Ru⁃Cl is Clion,and the sixth coordination position in complex Ru⁃NCME is N atom in acetonitrile molecule.The interaction of the two complexes with various amino acids and DNA was studied by UV⁃Vis absorption and fluorescence spectros⁃copy.It was found that the complexes with long alkyl chains and rigid coordination planes had an obvious specific recognition effect on DNA.Further study found that they could bind DNA in the way of insertion and electrostatic.Both results of the experiments and the theoretical calculation were consistent.CCDC:1063055,Ru⁃Cl;1577329,Ru⁃NCME.