猪轮状病毒GZAS2020株与NX株VP7蛋白的结构预测和抗原表位分析

Structural Prediction and Epitope Analysis of VP7 Protein of Porcine Rotavirus GZAS2020 and NX

为了解猪轮状病毒(PoRV)VP7蛋白的生物信息学信息,运用Expasy-ProParam、YinOYang-1.2、TMHMM-2.0、ABCpred和IEDB等软件,预测分析PoRV临床分离株GZAS2020和疫苗株NX VP7蛋白的基本理化性质、结构功能和B细胞抗原表位差异。结果显示:两种VP7蛋白均属于稳定蛋白、亲水性蛋白、跨膜蛋白和非分泌性蛋白;分离株与疫苗株的O糖基化位点数分别为55和62,N糖基化位点数均为1,磷酸化位点数分别为29和30;亚细胞定位均位于质膜,均以α-螺旋和无规则卷曲为主要结构,延伸和β-转角贯穿其中;GZAS2020株的B细胞抗原表位位于67~72 aa(PYANSTT)、97~100 aa(KWTE)、176~179 aa(QQTD)、276~283 aa(TTAPQTER),NX株位于96~99 aa(TKWK)、176~182 aa(QQTDEAN)、274~279 aa(DPTTTP)、310~317 aa(MSKRSRSL),两者在97~100、176~179、276~279 aa处存在相同的B细胞抗原表位,但GZAS2020株在无规则卷曲位置(67~72 aa)存在独特的B细胞抗原表位。以上结果说明,疫苗株NX与贵州省流行株GZAS2020在主要抗原蛋白上存在差异。

In order to learn the bioinformatics information of VP7 protein of porcine rotavirus(PoRV),the differences of general physicochemical properties,structural functions and B-cell epitopes of the clinical isolate GZAS2020 and the vaccine strain NX VP7 protein were predicted and analyzed by using Expasy-ProParam,YinOYang-1.2,TMHMM-2.0,ABCpred,IEDB and other software.The results showed that both the VP7 proteins belonged to stable proteins,hydrophilic proteins,transmembrane proteins and non-secretory proteins;the number of O-glycosylation sites in GZAS2020 and NX were 55 and 62,respectively,that of N-glycosylation site was 1,and that of phosphorylation sites was 29 and 30,respectively;all subcellular localization was located in the plasma membrane,with main structure ofα-helix and irregular coil,through which the extension andβ-turn run;the B-cell epitopes of the GZAS2020 strain were located at 67-72 aa(PYANSTT),97-100 aa(KWTE),176-179 aa(QQTD)and 276-283 aa(TTAPQTER),while those of NX were at 96-99 aa(TKWK),176-182 aa(QQTDEAN),274-279 aa(DPTTTP)and 310-317 aa(MSKRSRSL),both of which were with same B-cell epitopes at 97-100,176-179 and 276-279 aa,but unique B-cell epitopes were observed in the former at the position with irregular coil.It was concluded that the major antigen proteins of NX was different from those of GZAS2020 prevalent in Guizhou Province.