气相色谱法测定中药制剂中11种挥发性成分的含量

Determination of 11 Volatile Components in Chinese Medicine Preparation by GC

目的:建立同步测定中药制剂中11种挥发性成分的气相色谱方法。方法:采用HP-Innowax毛细管柱(30 m×0.25 mm×0.15 μm);柱温:初始温度60 ℃,保持2 min,然后以20 ℃/min升温至200 ℃;进样口温度:200 ℃;检测器温度:220 ℃;载气为氮气;流速1.0 mL/min;分流比10∶1。结果:11种挥发性成分在相应的质量浓度范围内线性关系良好,相关系数(r)为0.999 1~0.999 8,3个加标水平的平均回收率为90.9%~104.8%,相对标准偏差(RSD)为2.4%~6.0%。结论:该方法重复性好、准确度高,可用于中药制剂中挥发性成分的测定。

Objective:To establish a gas chromatography method for simultaneous determination of 11 volatile components.Methods:The HP-Innowax capillary column (30 m×0.25 mm×0.15 μm) was used.The column temperature:60℃,held for 2 min,increased at a rate of 20 ℃/min to 200 ℃.The inlet temperature was 200 ℃ and the detector temperature was 220 ℃.Nitrogen was selected as the carrier gas with a flow rate of 1.0 mL/min and the split flow ratio was 10∶1.Results:There were good linear relationships for the analytes in corresponding concentration ranges,with their correlation coefficients (r) of 0.999 1-0.999 8.The average recoveries at three spiked levels ranged from 90.9% to 104.8%,with relative standard deviations (RSD) of 2.4%-6.0%.Conclusion:The method is reproducible and accurate.It can be used to determine volatile components in Chinese medicine preparation.