miRNA-451和钙结合蛋白39在非小细胞肺癌患者中的表达及临床意义

Expression and clinical significance of miRNA-451 and calcium binding protein 39 in non-small cell lung cancer patients

目的探讨微小RNA(miRNA)-451和钙结合蛋白39(CAB39)在非小细胞肺癌(NSCLC)患者中的表达及临床意义。方法收集180例NSCLC患者的NSCLC组织和对应的癌旁组织,采用实时荧光定量聚合酶链反应检测miRNA-451相对表达量,采用免疫组织化学染色法检测CAB39表达情况。采用多因素Logistic回归模型分析NSCLC患者NSCLC组织中miRNA-451和CAB39表达情况的影响因素。比较不同miRNA-451、CAB39表达情况NSCLC患者的预后。结果NSCLC组织中miRNA-451相对表达量和CAB39阳性表达率均明显低于癌旁组织,差异均有统计学意义(P<0.01)。肿瘤直径>3 cm、有淋巴结转移、临床分期为Ⅲ~Ⅳ的NSCLC患者NSCLC组织中miRNA-451高表达率和CAB39阳性表达率均低于肿瘤直径≤3 cm、无淋巴结转移、临床分期为Ⅰ~Ⅱ期的患者,差异均有统计学意义(P<0.05)。多因素Logistic回归分析结果显示,淋巴结转移情况、临床分期均是NSCLC患者NSCLC组织中miRNA-451和CAB39表达情况的独立影响因素(P<0.05)。miRNA-451低表达患者的中位生存时间短于miRNA-451高表达患者,CAB39阴性表达患者的中位生存时间短于CAB39阳性表达患者,差异均有统计学意义(P<0.05)。结论NSCLC组织中miRNA-451和CAB39表达水平均明显低于癌旁组织,miRNA-451低表达和CAB39阴性表达可能会促进肿瘤生长、淋巴结转移,有望成为NSCLC患者治疗效果及预后的评估指标。

Objective To investigate the expression and clinical significance of microRNA(miRNA)-451 and calcium binding protein 39(CAB39)in patients with non-small cell lung cancer(NSCLC).Method The NSCLC tissues and corresponding paracancerous tissues from 180 NSCLC patients were collected.The relative expression of miRNA-451was detected by real-time fluorescence quantitative polymerase chain reaction.The expression of CAB39 was detected by immunohistochemical staining.Multivariate Logistic regression model was constructed to analyze the influencing factors for miRNA-451 and CAB39 expression in NSCLC tissues of NSCLC patients.The prognosis of NSCLC patients with different expression of miRNA-451 and CAB39 were also compared.Result The relative expression of miRNA-451 and the positive rate of CAB39 in NSCLC tissues were significantly lower than those in paracancerous tissues,and the differences were statistically significant(P<0.01).The high expression rate of miRNA-451 and the positive rate of CAB39 in NSCLC tissues of NSCLC patients with tumor diameter>3 cm,lymph node metastasis,and clinical stage Ⅲ-Ⅳ were lower than those with tumor diameter≤3 cm,no lymph node metastasis,and clinical stage Ⅰ-Ⅱ,and the differences were statistically significant(P<0.05).Multivariate Logistic regression analysis showed that lymph node metastasis and clinical stage were independent risk factors for miRNA-451 and CAB39 expression in NSCLC tissues of NSCLC patients(P<0.05).The median survival time of patients with miRNA-451 low-expression was shorter than that of the miRNA-451high-expression,and the median survival time of the patients with CAB39 negative expression was shorter than that of the CAB39 positive expression,and the differences were statistically significant(P<0.05).Conclusion The expression levels of miRNA-451 and CAB39 in NSCLC tissues were significantly lower than those in paracancerous tissues.Downregulation of miRNA-451 and CAB39 negative expression may promote tumor growth and lymph node metastasis,which is expected to be evaluation indicators for the effect and prognosis of NSCLC patients.