血清miR-98-5p、TRAF6 mRNA表达与脓毒症并发肺损伤的关系

Relationships between serum miR-98-5p and TRAF6 mRNA expression and sepsis complicated with lung injury

目的 探讨血清微小RNA-98-5p(miR-98-5p)、肿瘤坏死因子受体相关因子6(TRAF6)mRNA表达与脓毒症并发肺损伤的关系。方法 选择脓毒症患者172例(观察组),其中并发肺损伤59例、未并发肺损伤113例,另选同期体检健康的志愿者56例(对照组),采集所有研究对象外周静脉血,离心留取血清,采用RT-qPCR法检测血清miR-98-5p、TRAF6 mRNA。比较两组血清miR-98-5p、TRAF6 mRNA表达,分析脓毒症患者血清miR-98-5p表达与TRAF6 mRNA表达的关系。采用多因素Logistic回归模型分析脓毒症并发肺损伤的危险因素。采用受试者工作特征(ROC)曲线分析血清miR-98-5p、TRAF6 mRNA表达对脓毒症并发肺损伤的预测价值。结果 观察组血清miR-98-5p相对表达量低于对照组,血清TRAF6 mRNA表达高于对照组(P均<0.05)。Pearson相关分析显示,脓毒症患者血清miR-98-5p表达与血清TRAF6 mRNA表达呈负相关关系(r=-0.768,P<0.01)。多因素Logistic回归分析显示,脓毒性休克、入住ICU时间延长、机械通气时间延长、血乳酸水平升高、脓毒症相关器官衰竭评估评分增加、血清TRAF6 mRNA表达升高为脓毒症并发肺损伤的独立危险因素,血清miR-98-5p表达升高则为其独立保护因素(P均<0.05)。ROC曲线分析显示,血清miR-98-5p、TRAF6 mRNA表达联合预测脓毒症并发肺损伤的曲线下面积高于血清miR-98-5p、TRAF6 mRNA表达单独(P均<0.05)。结论 脓毒症并发肺损伤患者血清miR-98-5p表达降低、血清TRAF6 mRNA表达升高;血清miR-98-5p、TRAF6 mRNA可作为预测脓毒症并发肺损伤的生物标志物。

Objective To investigate the relationships of the expression of serum microRNA-98-5p(miR-98-5p)and tumor necrosis factor receptor-associated factor 6(TRAF6) with sepsis complicated with lung injury.Methods Totally172 patients with sepsis(observation group),including 59 patients with lung injury and 113 patients without lung injury,and 56 healthy volunteers(control group) who underwent physical examination during the same period were selected.Peripheral venous blood was collected and centrifuged to obtain the serum.The expression of miR-98-5p and TRAF6 mRNA in serum was detected by RT-qPCR.The serum miR-98-5p and TRAF6 mRNA expression levels were compared between the two groups.The relationship between serum miR-98-5p expression and TRAF6 mRNA expression in sepsis patients was analyzed.Multivariate Logistic regression model was used to analyze the risk factors for sepsis complicated with lung injury.Receiver operating characteristic(ROC) curve was used to analyze the value of serum miR-98-5p and TRAF6 mRNA expression in predicting sepsis complicated with lung injury.Results The relative expression of serum miR-98-5p in the observation group was lower than that in the control group,and the serum TRAF6 mRNA expression was higher than that in the control group(both P<0.05).Pearson correlation analysis showed that serum miR-98-5p expression in sepsis patients was negatively correlated with serum TRAF6 mRNA expression(r=-0.768,P<0.01).Multivariate Logistic regression analysis showed that septic shock,prolonged intensive care unit time,prolonged mechanical ventilation time,increased blood lactate,increased sepsis-related organ failure assessment score,and increased serum TRAF6 mRNA expression were independent risk factors for sepsis complicated with lung injury,while increased serum miR-98-5p expression was an independent protective factor(all P<0.05).ROC curve analysis showed that the area under the curve of serum miR-98-5p and TRAF6 mRNA expression combined in predicting sepsis complicated with lung injury was higher than that of serum miR-98-5p and TRAF6 mRNA expression alone(both P<0.05).Conclusion The decreased expression of serum miR-98-5p and the increased expression of serum TRAF6 mRNA are closely related to sepsis complicated with lung injury,and can be used as biomarkers to predict sepsis complicated with lung injury.