固相萃取-气质联用测定黄瓜中62种农药残留

Determination of 62 pesticide residues in cucumber by GC-MS combined with solid phase extraction technology

该文利用气相色谱串联质谱法,结合固相萃取技术建立黄瓜中62种农药残留检测方法。样品提取后,经Envi-18柱及Envi-Carb柱及Sep-Pak柱串联柱净化后,浓缩氮吹近干,正己烷定容后,采用气相色谱-质谱联用法测定,外标法定量。62种农药在基质中的线性范围良好;以三个添加水平测定样品的回收率和RSD,加标回收率均在60.0%~120.0%之间,相对标准偏差为1.5%~15.3%,该方法准确、高效、灵敏,且净化效果好,为检测黄瓜中目标物提供参考。该方法可以满足一般实验室检测需求,也为其他果蔬中农药残留检测提供方法借鉴。

In this paper, a method for simultaneous determination of 62 pesticide residues in cucumber by gas chromatography tandem mass spectrometry combined with solid phase extraction was established. After extraction, the samples were purified by ENvi-18 column, ENvi-Carb column and SEP-PAK column, and the concentrated nitrogen was blown nearly dry. After volume determination of n-hexane, the samples were determined by gas chromatography-mass spectrometry and quantified by external standard method. The linear range of 62 pesticides in the substrate was good, and the detection limit reached the current pesticide residue limit standard in China.The recovery rate and RSD of the samples were measured at three additive levels. The recoveries of most pesticide compounds were between 60.0% and 120.0%, and the relative standard deviation was between 1.5% and 15.3%. The method is accurate, efficient, sensitive, and has good purification effect. It can be used as a reference for the detection of target compounds in cucumber. which can meet the actual needs of general laboratory testing, and also provides a method for the detection of pesticide residues in other fruits and vegetables.