摘要
Mevalonate pyrophosphate decarboxylase is a kind of key enzyme in the terpenoid synthesis pathway in Eleutherococcus senticosus.The results of bisulfite sequencing showed that there were three kinds of samples with a low(0.68%),medium(0.72%)and high(0.79%)DNA methylation ratio in the promoter of MDD in E.senticosus,respectively.The transcriptome sequencing results showed that the expression of MDD in E.senticosus was significantly up-regulated in the types with low DNA methylation ratios of MDD(P<0.05).There was a significant negative correlation between the saponin content in E.senticosus and the DNA methylation ratio of MDD promoter(P<0.05).The screening results of differentially expressed transcription factors among the three groups with different DNA methylation ratio showed that a total of 4 transcription factors could bind to 6 CpG sites.Protein-protein interaction analysis showed that E.senticosus MDD could interacted with other key enzymes in the process of terpenoid synthesis.In addition,it was found that the DNA methylation of MDD promoter was mainly regulated by DNA methyltransferase.These results demonstrated that under the action of DNA methyltransferase,the changes of DNA methylation of MDD promoter could regulate its own expression level by affecting the combination ability of transcription factors,and then affected the expression of other genes in terpenoid synthesis pathway.The synthesis and accumulation of saponins in E.senticosus was also changed.
基金
funded by Natural Science Foundation of Hebei Province,Grant No.H2020209302 and Hebei Education Department Sponsored Scientific Research Projects,Grant No.ZD2019075.
