Oseltamivir decorated gold nanorods for visible rapid detection of influenza virus

奥司他韦修饰纳米金棒快速可视化检测流感病毒

In the present study,we developed a novel colorimetric strategy for the visible rapid detection of the influenza virus based on the unique optical properties of gold nanorods.Specifically,lipoic acid-modified Oseltamivir(OS),which is a strong neuraminidase inhibitor and used as the first-line drug in the treatment of influenza,was synthesized and further attached to the surface of gold nanorods(OS-LA-GNRs).The absorption band and density of OS-LA-GNRs were changed with the decreasing distance between the nanoparticles induced by the target-specific aggregation via strong neuraminidase–OS binding.All of these could be visible with the naked eyes and measured by UV-visible spectrophotometry.The results showed that our system had a comparable limit of detection(LOD)to the commercial colloidal gold stripes,making it feasible for wide diagnostic applications.

利用纳米金棒表面等离子共振吸收峰位置与强度随纳米粒子间距离变化的光学特性,使用与流感病毒表面神经氨酸酶具有强结合的抗流感一线药物奥司他韦的硫辛酸衍生物对纳米金棒进行修饰,借助其与流感病毒孵育前后金纳米颗粒聚集导致的颜色变化,使用肉眼及可见分光光度计对流感病毒进行快速可视化检测。结果表明,其检测限与现有的基于流感病毒核蛋白抗原-抗体相互作用的胶体金试纸条检测限相当,其廉价稳定的特性,具备进一步发展为新型流感检测试剂盒的潜力。