不同饮食模式对CUMS诱导的小鼠焦虑抑郁样行为的影响

Effects of different dietary patterns on anxiety and depression-like behavior induced by CUMS in mice

为探究不同饮食模式对小鼠焦虑抑郁样行为的影响,采用慢性不可预见性温和应激(chronic unpredictability mild stress,CUMS)模型,将C57BL/6小鼠随机分为正常饮食组(normal diet,ND)、正常饮食应激组(normal diet stress,NDS)、高脂饮食组(high fat diet,HFD)和高脂饮食应激组(high fat diet stress,HFDS),对小鼠的行为、体质量、脂质和神经递质等指标进行测定。结果显示,与ND组相比,HFD组小鼠糖水偏好显著降低,旷场试验中总运动距离、进入中心区域次数和跨格次数降低,HFDS组部分行为学指标较HFD组更低,且HFDS组部分行为学指标低于NDS组;HFD组体质量、体脂比、总胆固醇(TC)和甘油三酯(TG)水平最高,CUMS显著降低高脂饮食和正常饮食小鼠的体脂比、TC和TG水平,且与NDS组相比,这些脂质指标水平在HFDS组更低。结果表明,高脂饮食易诱导焦虑抑郁样行为,且高脂饮食会加重CUMS诱导的焦虑抑郁样行为,其机制可能与脂质代谢、中枢神经递质代谢途径有关。

Chronic unpredictability mild stress(CUMS)model was used to investigate the effects of different dietary patterns on anxiety and depression-like behaviors in mice.C57BL/6 mice were randomly divided into normal diet group(ND),normal diet stress group(NDS),high fat diet group(HFD)and high fat diet stress group(HFDS).The behavior,body weight,lipid profiles and neurotransmitters of mice were measured.The results showed that compared with the ND group,the sucrose preference of HFD group was significantly decreased,and the total movement distance,the number of entering the central area and the number of crossing squares were decreased in the open field test.Some behavioral indexes of HFDS group were lower than those of HFD group,and some behavioral indexes in HFDS group were lower than those in NDS group.The body weight,adipose/weight,TC and TG levels of HFD group were higher than those of the other groups,and CUMS significantly reduced the adipose/weight,TC and TG levels of the high-fat diet and normal diet mice,and these lipid indexes were lower in HFDS group than in NDS group.The contents of 5-HT and NE in HFD group were lower than those in ND group.CUMS significantly decreased the levels of 5-HT and NE in serum and hippocampus of high-fat diet and normal diet mice,and the levels of neurotransmitters in HFDS group were lower than that in NDS group.The results suggested that high-fat diet can easily induce anxiety and depression-like behaviors,and aggravate CUMS-induced anxiety and depression behaviors,which may be related to lipid metabolism and central neurotransmitter metabolism pathways.