液质联用法测定不同地域种植的“华墨香5号”黑米中花青苷和酚酸类化合物含量

Determing contents of anthocyanin and phenolic acid compounds in black rice variety‘Huamoxiang 5’grown in different locations with HPLC-MS

为研究不同种植地域对“华墨香5号”黑米中花青苷和酚酸类化合物含量的影响,在武汉、西安、陵水、宁波等14个地区种植了该黑米品种,通过高压液相色谱串联质谱(HPLC-MS/MS)测定不同种植地域来源的“华墨香5号”样品中靶向代谢物,以外标法进行定量分析。结果显示,不同地域种植的“华墨香5号”黑米中花青苷和酚酸类化合物含量存在显著性差异,其中来自西安地区样品检出量最高,含矢车菊素-3-O-葡萄糖苷2.855 mg/g、芍药色素-3-O-葡萄糖苷0.491 mg/g、原儿茶酸0.088 mg/g;黄石地区样品检出量最低,分别为0.865、0.162、0.048 mg/g。表明黑米中花青苷和酚酸类化合物积累显著受到种植环境影响,为生产高营养价值黑米,需针对特定品种筛选合适的种植地域。

‘Huamoxiang 5’is an excellent black rice variety developed by Huazhong Agricultural University.We planted‘Huamoxiang 5’in 14 locations including Wuhan,Xi’an,Lingshui,and Ningbo etc to study the effects of planting locations on the contents of anthocyanins and phenolic acid compounds.The rice flour samples of‘Huamoxiang 5’were extracted and analyzed by High Pressure Liquid Chromatography-tandem Mass Spectrometry(HPLC-MS),and the data were quantitatively determined with the external standard method.The results showed that the contents of anthocyanins and phenolic acid compounds in‘Huamoxiang 5’varied significantly among samples collected from different planting locations.Among them,the contents of anthocyanins and phenolic acid compounds in the sample planted in Xi’an was the highest,with the content of cyanidin-3-O-glucoside,peonidin-3-O-glucoside and protocatechuic acid up to 2.855 mg/g,0.491 mg/g and 0.088 mg/g,respectively.The contents of anthocyanins and phenolic acid compounds in the sample in Huangshi was the lowest,with the content of cyanidin-3-O-glucoside,peonidin-3-O-glucoside and protocatechuic acid of 0.865 mg/g,0.162 mg/g,and 0.048 mg/g,respectively.It is indicated that the contents of anthocyanins and phenolic acid compounds in black rice are significantly affected by the planting environment.Thus,selecting suitable planting locations for specific black rice varieties is important to produce the black rice with high nutritional value.