化学发光光纤免疫传感器的研制及其对谷物中呕吐毒素的快速检测

Development of a chemiluminescent optical fiber immunosensor and its application in rapid detection of deoxynivalenol in grains

为实现谷物中呕吐毒素(deoxynivalenol,DON)的快速、灵敏检测,采用生物传感和化学发光技术相结合的方式,研制应用于现场快速检测谷物中呕吐毒素的化学发光光纤免疫传感器,并搭建用于化学发光信号检测的便携式传感平台。整个检测平台集成到一个32 cm×15 cm×25 cm的黑色避光盒中,质量小于3 kg。结果显示:所研制的化学发光光纤免疫传感器在0.1~1200 ng/mL呈现出良好的线性关系,检出限为62.7 pg/mL;功能化的光纤探针能够稳定保存30 d且具有良好的特异性和抗干扰性;其应用于谷物样品的呕吐毒素检测回收率为81.00%~107.33%,相对标准偏差小于8.69%。结果表明,该方法较ELISA法的灵敏度提高了30倍,线性范围拓宽了3个数量级,适合应用于谷物样品中呕吐毒素的现场快速检测。

Deoxynivalenol(DON),as a trichothecenes metabolite produced by Fusarium,widely exists in grains including wheat,rice and corn,which seriously threatens human health.Therefore,based on the theoretical guidance of biochemistry,immunology and materials science,we constructed a chemiluminescent fiber optic immunosensor for the on-site rapid detection of DON in grains by using a combination of biosensing recognition and chemiluminescence technology.At the same time,a portable sensing platform for detecting the chemiluminescence signal was integrated into a black light-proof cassette of 32 cm×15 cm×25 cm,and the weight was less than 3 kg.The results showed that Fourier transform infrared,energy dispersive spectrometry and atomic force microscopy confirmed the successful preparation of fully antigenicized fiber probes.In addition,the constructed chemiluminescence optical fiber immunosensor had a good linear relationship between 0.1-1200 ng/mL,with the detection limit of 62.7 pg/mL.The functionalized optical fiber probe can be stably stored for 30 days and has good specificity and anti-interference.The recovery rate of DON applied to grain samples was 81.00%-107.33%,and the relative standard deviation was less than 8.69%.Compared with ELISA,the sensitivity of this method was increased by 30 times,and the linear range was broadened by 3 orders of magnitude,indicating that it is suitable for the on-site rapid detection of DON in grain samples.