摘要
目的建立高效液相色谱法同时测定华北獐牙菜中獐牙菜苦苷、龙胆苦苷、当药苷、芒果苷、异荭草素的含量。方法采用Waters SunFire C_(18)柱(4.6 mm×250 mm,5μm),甲醇-0.05%磷酸溶液为流动相进行梯度洗脱,流速1.0 mL·min^(-1),检测波长254 nm,进样量10μL,柱温30℃。结果獐牙菜苦苷、龙胆苦苷、当药苷、芒果苷、异荭草素进样量分别在0.0546~1.0930μg(r=0.9993)、0.1910~3.8200μg(r=0.9996)、0.1089~2.1780μg(r=0.9995)、0.0584~1.1690μg(r=0.9999)、0.0528~1.0570μg(r=0.9992)与峰面积呈良好的线性关系,加样回收率分别为97.62%、97.04%、97.68%、95.66%、96.75%,RSD分别为1.3%、0.79%、0.53%、0.79%、0.92%(n=6);3批样品中龙胆苦苷含量最高。结论该方法简便可行,精密度高,重复性好,可用于华北獐牙菜的质量控制。
Objective To establish an HPLC method for the simultaneous determination of swertiamarin,gentiopicroside,sweroside,mangiferin and isoorientin in Swertia wolfangiana Grun.Methods Waters SunFire C_(18) column(4.6 mm×250 mm,5μm)was used with methanol-0.05% phosphoric acid solution as the mobile phase at 1.0 mL·min^(-1).The detection wavelength was 254 nm,the sample volume was 10μL,and the column temperature was 30℃.Results The linearity for swertiamarin,gentiopicroside,sweroside,mangiferin and isoorientin were 0.0546~1.0930μg(r=0.9993),0.1910~3.8200μg(r=0.9996),0.1089~2.1780μg(r=0.9995),0.0584~1.1690μg(r=0.9999),and 0.0528~1.0570μg(r=0.9992).The recoveries were 97.62%,97.04%,97.68%,95.66%,and 96.75%.RSDs were 1.3%,0.79%,0.53%,0.79%,and 0.92%(n=6).Gentiopicroside had the highest content among the 3 samples batches.Conclusion The method is simple,feasible,accurate and reproducible,which can be used for the quality control of Swertia wolfangiana Grun..
作者
刘学良
刘安平
李维业
石春兰
问静
王永晶
祁彦凯
王开祥
陈鹏
韩达斌
刘海青
LIU Xue-liang;LIU An-ping;LI Wei-ye;SHI Chun-lan;WEN Jing;WANG Yong-jing;QI Yan-kai;WANG Kai-xiang;CHEN Peng;HAN Da-bin;LIU Hai-qing(Qinghai Center for Drug Evaluation and Inspection,Xining 810007;Centre for Food and Drug Control of Xining,Xining 810007)
出处
《中南药学》
CAS
2022年第8期1919-1922,共4页
Central South Pharmacy
基金
青海省基础研究项目(No.2020-ZJ747)。