三七皂苷R1上调miR-132对人下咽鳞癌FaDu细胞增殖、迁移、侵袭的抑制作用

NGR1 inhibits proliferation,migration,and invasion of FaDu cells through up-regulating miR-132 expression

目的探讨三七皂苷R1(NGR1)对人下咽鳞癌FaDu细胞中miR-132表达的影响及其抗肿瘤作用机制。方法以0、75、150、300μmol/L NGR1处理FaDu细胞,并分别标记为Control组、75μmol/L NGR1组、150μmol/L NGR1组、300μmol/L NGR1组。采用MTT法检测各组细胞增殖能力,Real-time PCR检测各组细胞miR-132相对表达量。再将FaDu细胞分为Control组(未处理)、NGR1组(给予150μmol/L NGR1)、miR-132 inhibitor-NC组(转染miR-132 inhibitor-NC)、miR-132 inhibitor组(转染miR-132 inhibitor)、miR-132 inhibitor-NC+NGR1组(转染inhibitor-NC后,给予150μmol/L NGR1处理)、miR-132 inhibitor+NGR1组(转染inhibitor后,给予150μmol/L NGR1处理),通过MTT、划痕以及Transwell侵袭试验检测抑制或上调miR-132后对FaDu细胞增殖、迁移以及侵袭能力的影响。结果Control组、75μmol/L NGR1组、150μmol/L NGR1组、300μmol/L NGR1组FaDu细胞增殖能力依次降低,细胞中miR-132的相对表达量依次升高,组间差异有统计学意义(P均<0.05)。与Control组相比,miR-132 inhibitor组FaDu细胞活力、迁移侵袭能力增强,NGR1组FaDu细胞活力、迁移、侵袭能力减弱(P均<0.05)。miR-132 inhibitor+NGR1组与NGR1组比较,FaDu细胞活力、迁移、侵袭能力增强(P均<0.05)。结论NGR1可能通过上调miR-132表达,抑制FaDu细胞的增殖、迁移以及侵袭。

Objective To investigate the effect of notoginseng saponin R1(NGR1)on the expression of miR-132 in human hypopharyngeal squamous cell carcinoma cell line FaDu,and to explore its possible anti-tumor mechanism.Methods FaDu cells were treated with 0,75,150 and 300μmol/L NGR1,and were labeled as the Control group,75μmol/L NGR1 group,150μmol/L NGR1 group and 300μmol/L NGR1 group,respectively.The proliferation ability of cells in each group was detected by MTT assay,and the relative expression of miR-132 in each group was detected by real-time PCR.FaDu cells were further divided into the Control group(no treatment),NGR1 group(given 150μmol/L NGR1),miR-132 inhibitor-NC group(transfected with miR-132 inhibitor-NC),miR-132 inhibitor group(transfected with miR-132 inhibitor),miR-132 inhibitor-NC+NGR1 group(150μmol/L NGRl treatment after transfection with inhibitor-NC),and miR-132 inhibitor+NGR1 group(150μmol/L NGR1 treatment after transfection with inhibitor),respectively.MTT,Scratch and Transwell invasion assays were used to detect the effects of inhibition or up-regulation of miR-132 on the proliferation,migration and invasion abilities of FaDu cells.Results The proliferation abilities of FaDu cells in the Control group,75μmol/L NGR1 group,150μmol/L NGR1 group and 300μmol/L NGR1 group decreased successively,and the relative expression of miR-132 in FaDu cells increased successively,and the differences were statistically significant(all P<0.05).Compared with Control group,the viability,migration and invasion abilities of FaDu cells in the miR-132 inhibitor group were enhanced,and the viability,migration and invasion abilities of FaDu cells in the NGR1 group were weakened(all P<0.05).Compared with the NGR1 group,the viability,migration and invasion of FaDu cells in miR-132 inhibitor+NGR1 group significantly increased(all P<0.05).Conclusion NGR1 may inhibit the proliferation,migration and invasion of FaDu cells by up-regulating the expression of miR-132.