高效液相色谱法同时测定白芷药材中的9种香豆素成分及其质量分析

Simultaneous Determination and Quality Analysis of Nine Coumarins in Angelica Dahurica by High Performance Liquid Chromatography

建立了白芷9种香豆素成分的定量测定方法,并用于化学统计分析,为白芷药材的质量控制提供参考。采用高效液相色谱法(HPLC)同时分析4个不同省份的23批白芷样品中9种香豆素成分,采用单因素方差分析(ANOVA)方法分析未硫熏和硫熏样品中9种香豆素成分的差异。采用系统聚类分析(HCA)和主成分分析(PCA)进行化学模式识别,对不同产地白芷药材和硫磺熏蒸情况进行分析。ANOVA结果表明硫熏和未硫熏样品中8种化学成分含量差异显著(P<0.05),硫磺熏蒸对白芷药材质量有较大影响。HCA和PCA结果均显示,23批白芷可分为3类,其中,硫熏样品聚为一类,河南、河北未硫熏样品聚为一类,四川、安徽未硫熏样品聚为一类。该实验所建立的含量测定结合化学统计学分析方法可用于白芷药材的综合质量评价。

To develop a quantitative method for simultaneous determination of nine coumarins of Angelica dahurica,which was used for chemical statistical analysis,so as to provide reference for the quality control of Angelica dahurica.Nine coumarins of 23 batches of Angelica dahurica originated from four provinces were analyzed by high performance liquid chromatography(HPLC).Then one-way analysis of variance(ANOVA)method was used to analyze the differences of nine coumarins between sulfur fumigation and no-sulfur fumigation samples.Hierarchical cluster analysis(HCA)and principal component analysis(PCA)were used to identify chemical patterns,which were able to identify the origins and smouldering sulphur of samples.The results of ANOVA showed that the contents of 8 chemical components were significant different(P<0.05)between the sulfur fumigated samples and unfumigated samples,so sulfur fumigation had a great influence on the quality of Angelica dahurica.Furthermore,the results of HCA and PCA showed that 23 batches of Angelica dahurica could be divided into three categories,and sulfur fumigate samples were clustered into one category,no-sulfur fumigate samples collected from Henan Province and Hebei Province were clustered into one category,and no-sulfur fumigate samples collected from Sichuan Province and Anhui Province were clustered into one category.The quantitative determination combined with chemical statistics method can be used in the quality control and evaluation of Angelica dahurica.