基于网络药理学探讨葶苈子治疗骨质疏松症的作用机制

The therapeutic mechanism of Tinglizi on osteoporosis based on network pharmacology

目的:通过网络药理学研究葶苈子治疗骨质疏松症的作用机制。方法:在中药系统药理学数据库与分析平台中检索葶苈子的有效成分及相关靶点;在Gene Cards数据库、OMIM数据库中检索骨质疏松症的疾病基因靶点;通过Perl脚本找到葶苈子与骨质疏松症的共同基因,使用String网站构建葶苈子与骨质疏松症之间蛋白质-蛋白质相互作用的关系网络;最后利用R软件对葶苈子与骨质疏松症交集基因进行基因本体论(GO)功能富集分析、京都基因与基因组百科全书(KEGG)通路富集分析。结果:从葶苈子中筛选出9个有效成分,作用于骨质疏松症127个靶点。蛋白质-蛋白质相互作用网络中的核心蛋白有蛋白激酶B(Akt Serine/Threonine Kinase 1,AKT1)、白细胞介素(Interleukin,IL)-6、血管内皮生长因子(Vascular Endothelial Growth Factor,VEGF)A、JUN蛋白、IL-1β。基因KEGG富集分析得到与骨质疏松症紧密相关通路主要有晚期糖基化终末产物(Advanced Glycation End Products,AGE)-晚期糖基化终末产物受体(Advanced Glycation End Product Receptor,RAGE)信号通路、脂质与动脉粥样硬化通路等154条通路。结论:葶苈子可通过多靶点、多通路治疗骨质疏松症,为研究葶苈子治疗骨质疏松症提供了理论依据。

Objective: To explore the therapeutic mechanism of Tinglizi(Semen Descurainiae) on osteoporosis based on network pharmacology. Methods: The effective components and related targets of Tinglizi were searched in Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform. The gene targets of osteoporosis were searched in GeneCards database and OMIM database.The common genes of Tinglizi and osteoporosis were identified by Perl script. The protein-protein interaction(PPI) network between Tinglizi and osteoporosis was constructed by String website. Finally, the GO functional enrichment analysis and KEGG pathway enrichment analysis were performed on the intersection genes of Tinglizi and osteoporosis by R language software. Results: Nine active components were screened from Tinglizi, which acted on 127 targets of osteoporosis. The core proteins of PPI network include AKT1, IL-6, VEGFA,JUN protein and IL-1β. The KEGG enrichment analysis showed that there were 154 pathways closely related to osteoporosis, including AGE-RAGE signaling pathway, lipid and atherosclerosis pathway and so on. Conclusion: Tinglizi can be used to treat osteoporosis through multi-target and multi-pathway, which provides a theoretical basis for researching Tinglizi in the treatment of osteoporosis.