紫薇LiAGL19基因的克隆、亚细胞定位及表达特性分析

Cloning,Subcellular Localization and Expression Characteristics Analysis of LiAGL19 Gene in Crape Myrtle

AGL19是MADS-box基因家族的成员,在花芽分化与花器官发育过程中起重要作用。为了探索AGL19基因在紫薇花器官发育过程中的作用,以紫薇‘湘韵’为试验材料,通过RT-PCR扩增技术,获得了紫薇AGL19基因序列,命名为LiAGL19基因(登录号为:MT364282)。利用生物信息学手段对其进行生物信息学分析,并在烟草中进行亚细胞定位。此外,采用实时荧光定量PCR检测了目的基因在紫薇不同组织、不同时期花芽的表达量。结果表明,紫薇Li AGL19基因的编码区全长657 bp,编码218个氨基酸,是一个无信号肽、无跨膜区、亲水性的不稳定蛋白,与玫瑰木具有较高的相似性。亚细胞定位显示LiAGL19基因定位于细胞核中。实时荧光定量PCR分析结果表明,LiAGL19基因在紫薇‘湘韵’长雄蕊中的表达量最高,在花萼、花瓣中的表达量最低;在紫薇花器官发育阶段,该基因在花芽中的表达量可能与AP1基因具有一定的相关性。本研究结果为研究LiAGL19基因在紫薇中的表达模式提供基础数据,为深入研究紫薇LiAGL19基因的功能提供一定的科学依据。

LiAGL19 is a member of the MADS-box gene family,and plays an important role in the process of flower bud differentiation and organ development of crape myrtle.In order to explore the AGL19 gene function of crape myrtle at floral organ development stage,the crape myrtle cultivar’Xiangyun’was selected as the experimental material,RT-PCR was employed to cloned a homologous gene of AGL19,named LiAGL19(GenBank Accession No.MT364282).Bioinformatics was utilized to analyze the protein,and subcellular localization was carried out in tobacco.In addition,real-time fluorescence quantitative PCR was employed to detect the expression of the gene in different tissues and flower buds at different stages.The results revealed that crape myrtle LiAGL19gene encoding a total length of 657 bp and 218 amino acids was an unstable protein without signal peptide,transmembrane and hydrophilic,which had a high similarity with Rhodamnia argentea.Subcellular localization showed that LiAGL19 gene was located in nucleus.RT-qPCR test demonstrated that expression of LiAGL19 gene was the highest in the long stamens,and the lowest in calyx and petal.During floral organ development stage,the expression of this gene in crape myrtle buds at different stages may be correlated with AP1 gene to some extent.The results of this study provide basic data for study of LiAGL19 gene expression pattern,and provided certain scientific basis for further study of LiAGL19 gene function in crape myrtle.