猪毛菜不同部位转录组特征分析

Analysis of Transcriptome Characteristics of Different Parts of Salsola collina Pall.

本研究以猪毛菜的地上部分和地下部分为实验材料,通过转录组测序分析探讨猪毛菜不同组织部位的基因表达特征和代谢物质形成有关的基因调控。测序数据分析结果显示获得192777条转录本序列。差异表达筛选得到14424个差异基因,其中上调表达基因6137个,下调表达基因8287个;GO功能富集分析得到14424个差异基因。差异基因与KEGG数据库进行比对,共有5169个差异基因注释在138个通路上,其中参与次生代谢途径共11条通路呈显著性富集。进一步分析黄酮类化合物合成和异喹啉生物碱生物合成通路中的差异基因和其对应的相关酶。随机选取了黄酮类化合物合成通路上的8个基因,通过RT-qPCR分析验证了转录组数据的准确性。本研究利用高通量测序技术和生物信息分析获得猪毛菜不同部位的转录组信息特征,为猪毛菜的药用成分研究和基因功能鉴定提供了科学依据。

The above-ground and underground parts of S.collina were used as experimental materials to analyze the gene expression characteristics of different tissue parts of S.collina and the gene regulation related to the formation of metabolites through transcriptome sequencing analysis in study.Sequencing data analysis results was showed,192777 transcript sequences were obtained from the transcriptome data of the above-ground and underground parts of S.collina,Differential expression screening resulted in 14424 differential genes,of which 6317were up-regulated and 8287 were down-regulated;14424 differential genes were obtained through GO functional enrichment analysis.The differential genes were compared with the KEGG database.A total of 5169 differential genes were annotated on 138 pathways,of which 11 pathways involved in metabolic pathways were significantly enriched.Further analyze the differential genes and their corresponding related enzymes in flavonoid synthesis and isoquinoline alkaloid biosynthesis pathways.Eight genes in the synthesis pathway of flavonoids were randomly selected,and the accuracy of the transcriptome data was verified by RT-qPCR analysis.The use of high-throughput sequencing technology and bioinformatics analysis to obtain the transcriptome information characteristics of different parts of S.collina provides a theoretical basis for the study of medicinal components and the identification of gene functions of S.collina.