基于多元统计的化湿败毒颗粒特征图谱分析及活性成分含量测定

Fingerprint and Multivariate Statistics Analysis of Huashibaidu Granule Gormula and Content Determination of Active Components

目的本文旨在于通过化湿败毒颗粒的主要药效成分表征,以建立化湿败毒颗粒的质量控制体系。方法通过UPLC法建立化湿败毒颗粒的特征图谱,并结合成分响应进行了大黄5种蒽醌类成分的含量测定。结果结合13味药的阴性对照,得到15批样品主要有11个特征峰,其色谱峰归属为赤芍(峰1、峰2)、厚朴(峰3、峰10、峰11)、甘草(峰4、峰8、峰9)、大黄(峰5、峰6、峰7),经过主成分分析得到4个主成分,其中峰8、峰9对主成分1影响较大,峰3、峰6对主成分2影响较大,峰5对主成分3影响较大,峰4对主成分4影响较大。并进一步得到结合蒽醌含量范围为0.179~0.316 mg·g^(-1)。结论本研究通过对化湿败毒颗粒的特征成分表征和活性成分评价,为说明化湿败毒颗粒的药效物质基础提供了依据。

OBJECTIVE To establish the quality control system of Huashibaidu granule gormula through the characterization of the main pharmacodynamic material basis.METHODS The fingerprints of Huashibaidu granules were established by UPLC,and the contents of five anthraquinones in rhubarb were determined combined with component response.RESULTS There were 11 characteristic peaks in 15 batches of samples combined with negative control of 13 herbs.The peak 1 and peak 2 were from Paeoni⁃ae Radix Rubra.The peak 3,peak 10 and peak 11 were all from Magnoliae Officinalis Cortex.The peak 4,peak 8 and peak 9 were all from Glycyrrhizae Radix et Rhizoma.The peak 5,peak 6 and peak 7 were all from Rhei Radix et Rhizoma.Four principal components were obtained by principal component analysis,and the peak 8 and peak 9 had great influence on principal component 1,the peak 3 and peak 6 had great influence on principal component 2,the peak 5 had great influence on principal component 3,the peak 4 had great influence on principal component 4.The contents were determined at the range of 0.179-0.316 mg·g^(-1).CONCLUSION In this study,the characterization of characteristic components and the evaluation of active components of Huashibaidu granule gormula are conducted,which lay a foundation for explaining the pharmacodynamic material basis of Huashibaidu granule gormula.