YAP在非贲门胃癌的表达及其基因多态性与YAP表达水平的关系

YAP expression in non-cardia gastric cancer and the relationship with its single nucleotide polymorphisms

目的检测Yes相关蛋白(YAP)在非贲门胃癌和癌旁正常胃组织中的表达差异,并分析YAP基因单核苷酸多态性(SNP)与YAP蛋白表达水平的关联性,初步探讨YAP与非贲门胃癌发生发展的关系,为胃癌的防治提供新思路。方法收集非贲门胃癌组织126例和癌旁正常组织104例,分别设置为病例组和对照组。采用免疫组织化学SP法检测非贲门胃癌组织和癌旁正常组织中YAP蛋白的表达水平。采用Taqman探针法进行基因分型。采用非条件性Logistic回归法计算比值比(OR值)及其95%可信区间(CI),用以评估SNP rs11225163和rs1820453在共显性、显性、超显性、隐性和附加5种遗传模式下与YAP蛋白表达水平的关联性。采用Haploview 4.2软件构建单体型,并分析单体型与YAP蛋白表达水平的关联性。结果YAP蛋白在非贲门胃癌组织中的表达水平显著高于癌旁正常胃组织,差异有统计学意义(P<0.001)。YAP蛋白表达水平与肿瘤分化程度呈负相关(P<0.05),与性别、年龄、淋巴结转移及肿瘤大小无关联(P>0.05)。SNP rs11225163和rs1820453在共显性、显性、超显性、隐性和附加5种遗传模式下与YAP蛋白表达水平无关联。结论YAP可能参与了非贲门胃癌的发生发展过程。

Objective To detect the differences in the expression of Yes-associated protein(YAP)in non-cardia gastric cancer and normal gastric tissue and analyze the associations between single nucleotide polymorphisms(SNP)in the YAP gene andits protein expression,and to preliminarily discuss the correlationbetween YAP and the development of non-cardia gastric cancer,so as to provide new ideas for the prevention and treatment of non-cardia gastric cancer.Methods 126 casesofnon-cardia gastric cancer tissues and 104 cases of paracancerous normal tissues were collected and set as case group and control group,respectively.Immunohistochemical SP assay was used to detect the expression of YAP in non-cardia gastric cancer tissues and adjacent normal tissues.Taqman method was used for genotyping.The unconditional logistic regression method was used to calculate the odds ratio(OR)and its 95%confidence interval(CI)to detect the associations between YAP SNPs and the expression of YAP protein under Codominant,Dominant,Overdominant,Recessive,and Log-additive genetic models.Results The expression of YAP protein in non-cardia gastric cancer tissues was significantly higher than that in normal gastric tissues(P<0.001).The expression level of YAP protein was negatively correlated with the degree of tumor differentiation(P<0.05).There was no correlationbetween the expression level of YAP protein and gender,age,lymph node metastasis and tumor size respectively(P>0.05).No associations were found between SNP rs11225163 and rs1820453 and the expression of YAP protein under Codominant,Dominant,Overdominant,Recessive and Log-additive genetic models.Haplotype was constructed by Haploview 4.2 software,and the association between haplotype and YAP protein expression was analyzed.Conclusion YAP may be involved in the development of non-cardia gastric cancer.