Eu^(3+)参比碳点比率荧光传感器构建及用于环丙沙星检测

Construction of Eu^(3+)Reference Carbon Dot Ratio Fluorescence Sensor and Its Application in Detection of Ciprofloxacin

以碳量子点(CQDs)和铕离子(Eu^(3+))为荧光基团,构建比率荧光探针(CQDs/Eu^(3+)),用于环丙沙星(CIP)的检测。在395 nm波长激发下,CQDs/Eu^(3+)在459 nm处存在较强的蓝色荧光峰,在616 nm处存在较弱的红色荧光峰,当CIP存在时,基于天线效应使得459 nm处的CQDS荧光强度增强。研究优化了CQDs和Eu^(3+)比例、响应时间、稳定性等实验条件,结果表明:该比率荧光探针对CIP具有特异性识别能力,荧光强度比(F_(459)/F_(616))与不同浓度CIP(0~7×10^(-6)mol/L)之间存在良好的线性关系,对CIP的最低检测限为0.3×10^(-6)mol/L,随着CIP的加入,成功用于牛奶中CIP检测。

A ratiometric fluorescent probe(CQDs/Eu^(3+))was constructed with carbon quantum dots(CQDs)and europium ions(Eu^(3+))as fluorophores for the detection of ciprofloxacin(CIP).At excitation at 395 nm wavelength,CQDs/Eu^(3+)has an intense blue fluorescent peak at 459 nm,and a weak red fluorescent peak at 616 nm in the presence of CIP.The fluorescence(FL)intensity of CQDS at 459 nm is enhanced based on the antenna effect.The major factors like ratio of CQDs and Eu^(3+),response time and stability were optimized,The results show that the ratio fluorescent probe has a specific recognition ability for CIP.And there is a linear relationship between the fluorescence intensity ratio(F_(459)/F_(616))and concentration of CIP(0-7×10^(-6)mol/L),and the lowest detection limit for CIP is 0.3×10^(-6)mol/L.This probe can be used for the detection of CIP in milk under the addition of CIP.