五首活血化瘀方对血瘀证新西兰兔血常规、血沉及心、肝脏器系数的影响

Effects of Five Recipes for Promoting Blood Circulation on Blood Routine,Erythrocyte Sedimentation Rate and Organ Coefficients of Heart and Liver in New Zealand Rabbits with Blood Stasis Syndrome

目的观察五首活血化瘀方对新西兰兔血瘀证血常规、血沉及心、肝脏器系数的影响,对比其作用差异,为临床选方用药提供实验依据。方法70只新西兰兔随机分为正常组10只,造模组60只。采用“饥饿+高脂饲料+肾上腺素”方法复制血瘀证模型。造模成功后将造模组随机分为模型组、血府组、丹参组、失笑组、活络组、桃红组。各复方组分别给予相应复方灌胃治疗,正常组和模型组予以同等剂量蒸馏水灌胃作为对照。治疗结束后腹主动脉取血检测血常规及血沉,空气栓塞处死后取心脏、肝脏计算脏器系数。结果与正常组比较,模型组红细胞计数(RBC)、红细胞压积(HCT)、血红蛋白(HGB)显著降低(P<0.01),平均红细胞体积(MCV)、平均红细胞血红蛋白含量(MCH)、平均红细胞血红蛋白浓度(MCHC)、白细胞计数(WBC)、中间细胞计数(Mid)、粒细胞计数(Gran)、肝脏系数显著升高(P<0.05或P<0.01),红细胞体积分布宽度变异系数(RDW-CV)、淋巴细胞计数(Lymph)、血小板计数(PLT)、血小板压积(PCT)、平均血小板体积(MPV)、血小板分布宽度(PDW)、血沉(ESR)、心脏系数差异无统计学意义。与模型组比较,丹参组、血府组、桃红组RBC显著升高(P<0.05或P<0.01);与模型组比较,桃红组HCT显著升高(P<0.05);与模型组比较,丹参组、血府组、活络组、桃红组MCV显著降低(P<0.05或P<0.01);与模型组比较,丹参组、血府组、失笑组、桃红组MCH显著降低(P<0.01);与模型组比较,丹参组、血府组、活络组MCHC显著降低(P<0.05);与模型组比较,血府组Mid、Gran显著降低(P<0.05);与模型组比较,活络组肝脏系数显著升高(P<0.05);与模型组比较,各复方组RDW-CV、HGB、PLT、PCT、MPV、PDW、WBC、Lymph、ESR、心脏系数差异无统计学意义。结论五首活血化瘀方调节红细胞、血红蛋白相关指标RBC、HCT、MCV、MCH、MCHC的作用力大小不同,桃红四物汤疗效最佳,其次为血府逐瘀汤;调节炎症反应相关指标Mid、Gran效果最优的是血府逐瘀汤。

Objective To observe the effects of five recipes for promoting blood circulation on blood routine,erythrocyte sedimentation rate,organ coefficients of heart and liver in New Zealand rabbits with blood stasis syndrome,and to compare their differences in order to provide experimental evidence for clinical selection of prescriptions and drugs.Methods Seventy New Zealand rabbits were randomly divided into a normal group(n=10)and a modeling group(n=60).The blood stasis syndrome model was replicated by the method of“starvation+high fat feed+adrenaline”.After successful modeling,the modeling group were randomly divided into model group,Danshen group,Xuefu group,Shixiao group,Huoluo group and Taohong group.Each compound group was given the corresponding compound gavage treatment,the normal group and the model group were given the same dose of distilled water as a control.After the treatment,blood was taken from the abdominal aorta to test blood routine and erythrocyte sedimentation rate.After air embolism,the heart and liver were taken to calculate the organ coefficient.Results Compared with the normal group,red blood cell count(RBC),hematocrit(HCT)and hemoglobin(HGB)in the model group were significantly reduced(P<0.01),mean corpuscular volume(MCV),mean corpuscular volume(MCH),mean corpuscular hemoglobin concentration(MCHC),white blood cell count(WBC),middle cell count(Mid),granulocyte count(Gran),and liver coefficient were significantly increased(P<0.05 or P<0.01),There was no significant difference in red blood cell distribution width coefficient of variation(RDW-CV),lymphocyte count(Lymph),platelet count(PLT),plateletcrit(PCT),mean platelet volume(MPV),platelet distribution width(PDW),erythrocyte sedimentation rate(ESR)and cardiac coefficient.Compared with the model group,the RBC of the Danshen group,the Xuefu group,and the Taohong group were significantly increased(P<0.05 or P<0.01);compared with the model group,the HCT of the Taohong group was significantly increased(P<0.05);compared with the model group,the MCV of the Danshen group,the Xuefu group,the Huoluo group and the Taohong group were significantly reduced(P<0.05 or P<0.01);compared with the model group,the MCH of the Danshen group,the Xuefu group,the Shixiao group and the Taohong group were significantly decreased(P<0.01);compared with the model group,The MCHC of the Danshen group,the Xuefu group and the Huoluo group were significantly reduced(P<0.05);Compared with the model group,the Mid and Gran of the Xuefu group was significantly reduced(P<0.05);compared with the model group,the liver coefficient of the Huoluo group was significantly increased(P<0.05);Compared with the model group,there was no statistically significant difference in RDW-CV,HGB,PLT,PCT,MPV,PDW,WBC,Lymph,ESR and cardiac coefficient in each compound group.Conclusion The effect of five recipes for promoting blood circulation on red blood cell and hemoglobin related indicators RBC,HCT,MCV,MCH,MCHC is different,and the Taohong Siwu Decoction(桃红四物汤)has the best curative effect,followed by Xuefu Zhuyu Decoction(血府逐瘀汤);Xuefu Zhuyu Decoction has the best effect on regulating Mid and Gran related indicators of inflammatory response.