D-柠檬烯亚急性经皮毒性研究

Subacute percutaneous toxicity study of D-limonene

目的研究D-柠檬烯重复经皮染毒对SD大鼠的毒性。方法40只SD大鼠随机分为4组:0、62.5、250和1000 mg/kg·bw·d,每组雌雄各5只,连续4周经皮染毒93%D-柠檬烯。观察各组大鼠的临床表现,检测各大鼠血液学指标、血生化指标。对大鼠主要脏器进行称重,计算脏器系数,组织病理学检查。结果各组大鼠试验期间均无死亡,精神状态、一般行为均无毒性表现;各剂量组大鼠的血液学指标、血生化指标与对照组比较,差异无统计学意义(P>0.05);各组间脏器重量和脏器系数差异无统计学意义(P>0.05);未发现与受试样品有关的组织病理学改变。结论93%D-柠檬烯短期重复经皮染毒(28 d)毒性试验对SD大鼠无明显毒性。

Objective:To study the toxicity of D-limonene on SD rat after short-term repeated transdermal exposure.Methods Forty SD rats were randomly divided into 62.5,250,1000 mg/kg·bw·d of 93%D-limonene and control group,with five males and five females in each group transdermal exposure of 93%D-limonene for four consecutive weeks.Clinical symptoms of each rats were observed during experiment.Hematological,blood biochemical indexes,organ weights,histopathological examinations of the rats were detected after D-limonene exposure.Results No death,changes in mental state and general behavior of rats in each group were observed during the test.Compared with the control group,there were no statistically significant differences in hematological,blood biochemical parameters,organ weights,organ coefficients.No histopathological changes associated with the sample were found.Conclusion No obvious toxicity to SD rat was found after 93%D-limonene exposure by derma for 28 days.