布氏乳杆菌L34-2对黄曲霉毒素脱毒条件优化

Optimization of Detoxification Conditions of AFB1 by Lactobacillus buchneri L34-2

本研究以甘肃地区酸菜中分离的一株黄曲霉毒素B脱毒率高达66.4%的布氏乳杆菌(Lactobacillus buchneri)L34-2为材料,以菌株对AFB脱毒条件及脱毒机制为研究内容。结果表明,脱毒最佳反应条件为发酵温度30 ℃,发酵液pH值4.0,接种比例50 mL/300 mL,氧气供应量60%,优化后菌株L34-2脱毒率达到85.7%,比优化前提高21.1%,影响脱毒效果最大的因素是发酵温度。

In this study,Lactobacillus buchneri L34-2,a strain of aflatoxin Bwith a detoxification rate of 66.4% isolated from sauerkraut in Gansu,was used as the material to study the detoxification conditions and detoxification mechanism of AFBby the strain.The results showed that the best reaction conditions for detoxification were fermentation temperature 30 ℃,pH 4.0 of fermentation broth,inoculum ratio 50 mL/300 mL,and oxygen supply 60%,and the detoxification rate of strain L34-2 reached 85.7% after optimization,which was 21.1% higher than that before optimization,and the factor that affected the detoxification effect most was fermentation temperature.