椰毒假单胞菌酵米面亚种检测方法研究进展

Research Progress on Detection Methods of Psedomonas cocovenenans subsp.farinofermentans

近年来,由椰毒假单胞菌酵米面亚种引起的食物中毒事件常有发生,其毒素米酵菌酸的致病性极强,死亡率高,引起人们的高度关注。本文主要从国标法中的微生物培养技术、基因测序技术、PCR技术(包含常规PCR和实时荧光PCR技术)、环介导等温扩增技术及微滴数字PCR技术等方面总结椰毒假单胞菌酵米面亚种的检测方法,并对该菌未来的研究方向进行展望。

In recent years,food poisoning incidents caused by Psedomonas cocovenenans subsp.farinofermentans have occurred frequently.The toxin of Psedomonas cocovenenans subsp.farinofermentans is highly pathogenic and has a high mortality rate,which has aroused great concern of the people.In this paper,the detection methods of Psedomonas cocovenenans subsp.farinofermentans were summarized from the aspects of microbial culture technology,gene sequencing technology,PCR technology(including conventional PCR and real-time fluorescence PCR),loop-mediated isothermal amplification technology and droplet digital PCR technology.Finally,the future research directions of Psedomonas cocovenenans subsp.farinofermentans were prospected.