芍药内酯苷通过抑制脊髓NLRP3炎症小体活化缓解神经病理性疼痛

Albiflorin Relieves Neuropathic Pain by Inhibiting the Activation of the Spinal Cord NLRP3 Inflammasome

目的探索芍药内酯苷缓解神经病理性疼痛的作用机制。方法将40只雄性SD大鼠随机分为假手术(Sham)组、模型(CCI)组、MCC950给药组和芍药内酯苷给药组,每组10只。采用慢性坐骨神经缩窄性损伤手术建立模型。术后1d各组开始给予相应药物,模型组给予等量生理盐水,各组连续给药至术后11d。采用Von Frey电子测痛仪检测大鼠机械性缩足反射阈值;采用BME-410C热痛仪检测大鼠热痛阈;采用免疫荧光法检测NLRP3表达水平;采用Caspase-1活性分析试剂盒检测脊髓Caspase-1活性;采用IL-1βELISA试剂盒检测脊髓IL-1β含量。结果与模型组相比,芍药内酯苷治疗组机械性痛阈与热痛阈显著提高(P<0.001,P<0.01),脊髓背角NLRP3表达水平、Caspase-1活性、IL-1β含量均有显著降低(P<0.01,P<0.001,P<0.05)。结论芍药内酯苷可通过抑制脊髓NLRP3活化缓解神经病理性疼痛。

Objective To explore the mechanism of paeonifl orin in the treatment of neuropathic pain.Methods Forty male SD rats were randomly divided into the sham group,the chronic Constrictive injury(CCI)group,the MCC950 group and the albifl orin(AF)group,10 rats in each group.The CCI model was established by CCI surgery.One day after operation,the corresponding drugs were given to each group and the model group was given the same amount of normal saline.The drugs were continuously given to each group until 11 days after operation.The paw withdrawal threshold(PWT)was measured by electronic Von Frey.The paw withdrawal latency(PWL)was measured by thermal pain stimulator.The caspase-1 activity was measured by caspase-1 activity assay kit.The IL-1βconcentration was measured by ELISA kit.Results Compared with the CCI group,the PWT and PWL of AF group were signifi cantly increased(P<0.001,P<0.01).NLRP3 level,Caspase-1activity and IL-1βcontent were signifi cantly decreased(P<0.01,P<0.001,P<0.05).Conclusion Albifl orin can effectively relieve neuropathic pain by controlling NLRP3 in spinal cord.