番茄总皂苷对小鼠高尿酸血症的调节作用

Modulating effects of tomato total saponin on uric acid in hyperuricemia mice

为研究番茄总皂苷对尿酸的调节作用,该文以番茄水提物为试材,利用次黄嘌呤和氧嗪酸钾以及尿酸和氧嗪酸钾建立高尿酸模型小鼠,考察番茄总皂苷对正常小鼠及高尿酸血症小鼠尿酸排泄量、血尿酸、尿素氮、肌酐、黄嘌呤氧化酶以及脏器指数的影响。结果表明:番茄总皂苷不影响正常小鼠血尿酸水平,正常组及番茄低、中、高剂量组血尿酸值分别为(170.4±36.7)、(178.3±69.7)、(175.5±42.1)、(185.3±72.6)μmol·L^(-1);番茄总皂苷对次黄嘌呤和氧嗪酸钾联合诱导的高尿酸血症小鼠可以降低血尿酸水平,降低黄嘌呤氧化酶活性,正常组、模型组及番茄高剂量组血尿酸值分别为(140.4±36.7)、(378.3±69.7)、(278.3±62.6)μmol·L^(-1),正常组、模型组及番茄低、中、高剂量组黄嘌呤氧化酶值分别为(1.2±0.3)、(1.8±0.2)、(1.6±0.2)、(1.5±0.3)、(1.3±0.4)U·g^(-1) liver;对尿酸和氧嗪酸钾联合诱导的高尿酸血症小鼠,可降低血尿酸水平,降低黄嘌呤氧化酶活性,正常组、模型组及番茄高剂量组血尿酸值分别为(98.8±21.8)、(455.6±78.8)、(333.7±68.7)μmol·L^(-1),正常组、模型组及番茄高剂量组黄嘌呤氧化酶值分别为(2.1±0.3)、(2.5±0.2)、(2.3±0.2)U·g^(-1) liver。综上结果表明,番茄总皂苷不影响正常小鼠血尿酸水平,但能降低高尿酸模型小鼠的血尿酸水平,其机制可能与降低黄嘌呤氧化酶活性有关。

In order to investigate modulating effect of total tomato saponin(TTS)on uric acid,we used hypoxanthine,oxonic acid potassium salt,and uric acid and potassium oxyzincate to establish hyperuric acid model mice,and investigated the effects of TTS on uric acid excretion,blood uric acid,urea nitrogen,creatinine,xanthine oxidase and organ index in normal mice and hyperuricemic mice.The experimental results were as follows:TTS did not affect the blood uric acid level in normal mice,and the blood uric acid values in the normal group and the low,medium and high dose groups of TTS were(170.4±36.7),(178.3±69.7),(175.5±42.1),(185.3±72.6)μmol·L^(-1),respectively.TTS could reduce blood uric acid level and xanthine oxidase activity in hypoxanthine and oxonic acid potassium salt combination-induced hyperuricemic mice,and the blood uric acid values in normal group,model group and TTS high dose group were(140.4±36.7),(378.3±69.7),(278.3±62.6)μmol·L^(-1),respectively.The xanthine oxidase values in the normal,model and TTS low,medium and high dose groups were(1.2±0.3),(1.8±0.2),(1.6±0.2),(1.5±0.3),(1.3±0.4)U·g^(-1) liver,respectively.TTS reduced blood uric acid levels and decreased xanthine oxidase activity in mice with hyperuricemia induced by the combination of uric acid and oxonic acid potassium salt,and the blood uric acid values in normal group,model group and TTS high dose group were(98.8±21.8),(455.6±78.8),(333.7±68.7)μmol·L^(-1),respectively.The xanthine oxidase values in the normal,model and TTS high dose groups were(2.1±0.3),(2.5±0.2),(2.3±0.2)U·g^(-1) liver,respectively.The above results suggest that TTS does not affect blood uric acid levels in normal mice,but can reduce blood uric acid levels in mice in a hyperuric acid model,and the mechanism may be related to the reduction of xanthine oxidase activity.