纤枝金丝桃化学成分研究

Chemical components of Hypericum lagarocladum

为了探究滇产植物纤枝金丝桃的物质基础,寻找活性化合物,该文用80%乙醇对纤枝金丝桃地上部分浸渍提取,应用HP-20大孔吸附树脂、硅胶、葡聚糖凝胶、半制备高效液相等色谱技术对纤枝金丝桃的化学成分进行分离纯化,根据波谱数据鉴定化合物的结构。结果表明:(1)从纤枝金丝桃中分离得到15个化合物,分别鉴定为attenuatumione G(1)、uralodin B(2)、chipericumin C(3)、2,5-二羟基-1-甲氧基氧杂蒽酮(4)、1,7-二羟基氧杂蒽酮(5)、1,7-二羟基-4-甲氧基氧杂蒽酮(6)、槲皮苷(7)、芹菜素-7-O-β-D-葡萄糖苷(8)、芹菜素-7-O-β-D-(6″-O-乙酰基)-葡萄糖苷(9)、木犀草素(10)、槲皮素(11)、白桦脂酸(12)、白桦脂酸甲酯(13)、白桦脂酮酸(14)、β-谷甾醇(15)。化合物1-14为首次从该植物中分离得到。(2)采用MTT法对化合物1-14进行体外抗乳腺癌活性测试,结果仅显示化合物3、6、13对2种乳腺癌细胞株MCF-7和MDA-MB-231有一定的抑制作用,其IC_(50)值为48.6~123.5μg·mL^(-1)。该研究结果对综合开发利用纤枝金丝桃资源具有理论和应用意义。

The purpose of this paper was to study the material basis and to find the bioactive chemical components of Hypericum lagarocladum.The aerial part of this species was extracted with 80%ethanol,and then the crude extract was isolated and purified by HP-20 macroporous adsorption resin column chromatography(CC),silica gel CC,Sephadex LH-20 CC and semi-preparative HPLC.The structures of isolated compounds were deduced by the spectroscopic data,as well as comparison with the previous literature data.The results were as follows:(1)Fifteen compounds were isolated from H.lagarocladum,and those compounds were identified as attenuatumione G(1),uralodin B(2),chipericumin C(3),2,5-dihydroxy-1-methoxyxanthone(4),1,7-dihydroxyxanthone(5),1,7-dihydroxy-4-methoxyxanthone(6),quercitrin(7),apigenin-7-O-β-D-glucopyranoside(8),apigenin-7-O-β-D-(6″-O-acetyl)-glucopyranoside(9),luteolin(10),quercetin(11),betulinic acid(12),betulinic acid methyl ester(13),betulonic acid(14),andβ-sitosterol(15).Compounds 1-14 are isolated from H.lagarocladum for the first time.(2)In vitro cytotoxicites of compounds 1-14 were evaluated using MTT method against MCF-7 and MDA-MB-231 cell lines.And only compounds 3,6 and 13 showed weak cytotoxicites with IC_(50) values ranging from 48.6 to 123.5μg·mL^(-1).These research results provide scientific theoretical and applied implications of H.lagarocladum for its comprehensive development and utilization.