多刺绿绒蒿WD40基因家族的鉴定及生物信息学分析

Identification and bioinformatics analysis of WD 40 gene family of Meconopsis horridula

WD40转录因子家族广泛参与调节植物生长、发育、次生代谢物积累和环境适应等过程。为了探究WD40家族在多刺绿绒蒿生长、发育和次生代谢物积累以及抗逆方面的作用,该研究基于全长转录组测序数据,鉴定了多刺绿绒蒿WD 40基因家族成员,并对这些基因及其编码的蛋白进行了生物信息学分析。结果表明:(1)共鉴定到19个WD 40基因,编码的蛋白均具有WD40结构域,氨基酸数目为109~758 aa,分子量介于11830~84130 Da之间,预测大多数蛋白定位在细胞核中且都为亲水性蛋白;(2)系统进化树分析表明多刺绿绒蒿与罂粟、博落回亲缘关系较近;(3)WD 40基因启动子区域均存在数量不等的激素或逆境响应元件,表明该家族基因可能参与植物生长、发育和次生代谢物积累等多种生物学进程的调节;(4)蛋白三级结构显示这些蛋白在进化过程中发生了不同程度的进化。这些结果可为深入研究多刺绿绒蒿WD 40基因家族在其响应逆境胁迫和次生代谢物积累等方面的具体机制提供前期基础。

WD40,as a transcription factor family,is widely involved in regulating plant growth,development,secondary metabolite accumulation and environmental adaptation.In order to explore the role of the WD40 family in the growth,development,secondary metabolite accumulation and tolerance,the WD 40 genes of Meconopsis horridula were identified in this study based on the full-length transcriptome sequencing data and conducted bioinformatics analysis of these genes and their encoded proteins.The results were as follows:(1)A total of 19 WD 40 genes were identified,and all the proteins included typical WD40 domain,the amino acid numbers and molecular weight of the protein encoded by WD 40 genes were 109-758 aa and within 11830-84130 Da,respectively,and most of the proteins localized in the nucleus,and all proteins belonged to hydrophilic protein;(2)Phylogenetic tree analysis showed that WD40 proteins of Meconopsis horridula were closely to Papaver somniferum and Macleaya cordata;(3)The promoter region of WD 40 gene contained different amounts of hormones or stress-response elements,suggesting that this family genes may be involved in the regulation of various biological processes,such as growth,development and secondary metabolite accumulation;(4)The tertiary structure of WD40 proteins showed that these proteins evolved in different degrees during the evolutionary process.These results can provide a preliminary basis for further research on the specific mechanism of WD 40 gene family in response to stress and secondary metabolite accumulation.