鞘柄菝葜药材质量标准研究

Quality standard of Smilax stans

对鞘柄菝葜药材根及根茎进行性状和显微鉴别,以及对薯蓣皂苷元和白藜芦醇进行定性、定量分析,按2015年版《中国药典(四部)》相关通则对水分、总灰分、浸出物进行检查,初步建立鞘柄菝葜药材的质量标准。结果表明:鞘柄菝葜药材性状与显微特征性强;薄层色谱(TLC)分离度好,薯蓣皂苷元和白藜芦醇在对应的斑点清晰;药材水分、总灰分、醇溶性浸出物的含量范围分别为3.72%~7.91%、1.54%~4.74%、5.08%~7.31%;薯蓣皂苷元在0.10~0.70 mg·mL^(-1)范围内与峰面积呈良好的线性关系,平均加样回收率为93.32%(RSD 2.69%);白藜芦醇在6.375~255μg·mL^(-1)范围内与峰面积呈良好的线性关系,平均加样回收率为96.45%(RSD 2.94%)。该研究建立的方法专属性强、重复性好,为其物种鉴别、质量控制及临床合理用药提供依据。

To preliminarily establish the quality standards of Smilax stans the traits and microscopic identification of the samples were carried out.Diosgenin and resveratrol were analyzed qualitatively and quantitatively.Water,total ash and alcohol soluble extract contents were checked according to the relevant general rules of Chinese Pharmacopoeia(PartⅣ).The results were as follows:Smilax stans had obvious characters and microscopic characteristics;The separation degree of thin-layer chromatography(TLC)was specific,the spots corresponding to diosgenin and resveratrol were clear;The range of water,total ash and alcohol soluble extract contents were 3.72%-7.91%,1.54%-4.74%,5.08%-7.31%,respectively;The linear range of diosgenin was 0.10-0.70 mg·mL^(-1) and the average added sample recovery was 93.32%(RSD 2.69%);The linear range of resveratrol was 6.375-255μg·mL^(-1) and the average added sample recovery was 96.45%(RSD 2.94%).The method has specificity and feasible,which is the basis for species identification,quality control and clinical rational application.