广州中心城区污水厂聚磷菌丰度差异及影响因素

Abundance Difference of Phosphate Accumulating Organisms in Wastewater Treatment Plants in Central Area of Guangzhou and Its Influencing Factors

为研究华南地区采用传统厌氧/缺氧/好氧(AAO)工艺和膜生物反应器(MBR)工艺(典型工艺为厌氧/缺氧/好氧/缺氧/膜池)的城市污水净化厂的生物除磷效能差异及其关键影响因素,采用16S rRNA基因扩增子测序技术对13座污水净化厂的24条生产线中聚磷菌Candidatus Accumulibacter及Tetrasphaera的相对丰度进行检测。结果表明,采用AAO和MBR工艺的污水厂中两种聚磷菌的丰度差异显著,AAO工艺中Candidatus Accumulibacter的平均相对丰度为MBR工艺的3倍;采用MBR工艺的污水厂中均未检测到Tetrasphaera。对水质参数、运行条件、除磷效能、工艺模式及聚磷菌菌群结构进行典型对应分析(CCA),结果显示,除进水碳磷比、厌氧池水力停留时间外,硝化液回流模式是导致聚磷菌丰度差异的重要因素。

To investigate the difference in biological phosphorus removal efficiency and key influencing factors of wastewater treatment plants(WWTPs)using traditional anaerobic/anoxic/oxic(AAO)process and membrane bioreactor(MBR)process(typical process is anaerobic/anoxic/oxic/anoxic/membrane tank)in South China,the relative abundance of phosphate accumulating organisms(PAOs)Candidatus Accumulibacter and Tetrasphaera in activated sludge samples from 24 process lines of 13 WWTPs was detected by 16S rRNA gene amplicon sequencing technology.There was a significant difference in abundance of the two kinds of PAOs in the WWTPs using AAO and MBR processes,and the average relative abundance of Candidatus Accumulibacter in AAO process was 3 times of that in MBR process.Tetrasphaera was not detected in the WWTPs using MBR process.Canonical correlation analysis was performed to correlate the wastewater quality parameters,operational conditions,phosphorus removal performance,process configuration and microbial community of PAOs.In addition to the influent carbon to phosphorus ratio and the hydraulic retention time of anaerobic tank,the reflux mode of nitrification liquid was an important factor leading to the abundance difference of PAOs.