对甲酚降解菌对自闭症小鼠行为及微生物群落-肠道-脑轴影响

Influence P-cresol Degrading Bacteria for Behavior and Microbiotagut-brain Axis of Mouse With Autism Spectrum Disorder

目的 探究对甲酚降解菌诱导自闭症小鼠行为变化及对微生物群落-肠道-脑轴的影响。方法 将2022年1—2月的20只孤独症谱系障碍模型(Black and Tan Bachyuric-T+tf/J,BTBRT+tf/J)近交系小鼠随机分为研究组(对甲酚降解菌干预组)10只和对照组(空白质粒大肠杆菌组)10只,选取10只昆明(Kunming,KM)小鼠为空白组。比较三组的行为学实验(旷场试验、理毛实验、大理石包埋实验及三箱实验)及微生物群落-肠道-脑轴指标[粪便活菌数、肠道组织过氧化氢酶(catalase,CAT)、丙二醛(malondialdehyde,MDA)及总抗氧化能力(total antioxidant capacity,TAC)、海马组织核因子相关因子-2 (NF-E2-related factor,2Nfr-2)及超氧化物歧化酶(superoxide dismutase,SOD)]。结果 空白组的旷场试验、理毛实验、大理石包埋实验及三箱实验显著优于研究组及对照组,研究组则显著优于对照组;空白组的粪便活菌数、肠道组织CAT及TAC、海马组织SOD显著高于研究组及对照组,研究组则显著高于对照组;肠道组织MDA及海马组织Nfr-2显著低于研究组及对照组,研究组显著低于对照组(P<0.05)。结论 对甲酚降解菌可改善自闭症小鼠的相关行为,可能为通过影响微生物群落-肠道-脑轴来达到影响疾病的目的。

Objective To investigate the behavior change of p-cresol degrading bacteria in mouse with autism spectrum disorder and influence for microbiota-gut-brain axis.Methods A total of 20 BTBRT+tf/J inbred strain mice from January to February 2022 were randomly divided into study group(p-cresol degrading bacteria group) 10 mice and control group(blank plasmid Escherichia coli group) 10 mice,10Kunming mouse were chosen as the blank group.Then the behavioral experiment(open field test,self-grooming test,marble embedding test and three-chambered test) and microbiota-gut-brain axis indexes(fecal viable count,intestinal tissue CAT,MDA and TAC,hippocampus tissue Nfr-2and SOD) of three groups were compared.Results The open field test,self-grooming test,marble embedding test and threechambered test results of blank group were significantly better than those of study group and control group,the results of study group were significantly better than those of control group;the fecal viable count,intestinal tissue CAT and TAC and hippocampus tissue SOD of blank group were significantly higher than those of study group and control group,the results of study group were significantly higher than those of control group;the intestinal tissue MDA and hippocampus tissue Nfr-2 were significantly lower than those of study group and control group,the results of study group were significantly lower than those of control group(P<0.05).Conclusion The p-cresol degrading bacteria can significantly improve the related behavior of mouse with autism spectrum disorder,and it may be the purpose of influencing diseases by affecting the microbial community gut brain axis.