黄芪中多种黄酮类成分的测定研究

Quantification of flavonoid compounds in Astragali Radix

目的:建立同时测定黄芪中毛蕊异黄酮葡萄糖苷、刺芒柄花苷、毛蕊异黄酮、山柰酚、异鼠李素和芒柄花素含量的超高效液相方法,并对结果数据进行初步分析。方法:使用Phenomenex Luna Omega C_(18)色谱柱(100 mm×2.1 mm, 1.6μm),以0.1%甲酸水溶液-甲醇为流动相,进行梯度洗脱,流速0.4 mL·min^(-1),柱温35℃,检测波长254 nm。结果:毛蕊异黄酮葡萄糖苷、刺芒柄花苷、毛蕊异黄酮、山柰酚、异鼠李素和芒柄花素色谱峰均得到了良好地分离,分别在0.83~8.33、0.21~2.10、0.07~0.72、0.06~0.58、0.04~0.40和0.04~0.41μg·mL^(-1)范围内线性良好,平均回收率结果为97.2%~101.6%,RSD为0.78%~2.4%。这6个黄酮类成分可能存在一定的规律。结论:方法简便、快速、准确,适用于黄芪中黄酮类成分的测定与分析。

Objective: To establish an UPLC method for simultaneous quantification of campanulin, onospin, calycosin, kaempferol, isorhamnetin and formononetin in Astragali Radix and analyze regular pattern of six flavonoids content data preliminarily. Methods: The analysis was performed on a Phenomenex Luna Omega C_(18)(100 mm×2.1 mm, 1.6 μm) column at 35 ℃ using 0.1% formic acid solution and methanol as the mobile phase at a flow rate of 0.4 mL·min^(-1)with detection wavelength of 254 nm. Results: The separation of campanulin, onospin, calycosin, kaempferol, isorhamnetin and formononetin was good and the six types of flavonoids in 64 samples were quantified precisely. The calibration curves were linear in the ranges of 0.83-8.33 μg·mL^(-1), 0.21-2.10 μg·mL^(-1), 0.07-0.72 μg·mL^(-1), 0.06-0.58 μg·mL, 0.04-0.40 μg·mLand 0.04-0.41 μg·mLfor campanulin, onospin, calycosin, kaempferol, isorhamnetin and formononetin, respectively. The mean recoveries of campanulin, onospin, calycosin, kaempferol, isorhamnetin and formononetin were 97.2%-101.6% with RSDs of 0.7%-2.4%. These six flavonoids might have some rules. Conclusion: This method is simple, rapid, accurate and is suitable for the analysis of flavonoids in Astragali Radix.