摘要
本研究采用胃蛋白酶水解信阳油茶饼粕蛋白得到粗多肽,经超滤分离获得>30 kDa、10~30 kDa、3~10 kDa和<3 kDa的四个多肽片段,其中3~10 kDa片段的抗氧化活性最高,采用Sephadex G-50葡聚糖凝胶层析法对该多肽片段进一步纯化,得到5个组分,其中P5的抗氧化活性显著高于其他组分(p<0.5),电泳结果表明纯化效果较好,P1~P5都有单一的条带,且在11 kDa以下。
In this study, pepsin was used to hydrolyze Xinyang Camellia oleifera cake protein to obtain crude polypeptides, and four polypeptide fragments >30 kDa, 10~30 kDa, 3~10 kDa and <3 kDa were obtained by ultrafiltration separation, among which the antioxidant activity of the 3~10 kDa fragment was the highest. The polypeptide fragment was further purified by Sephadex G-50 dextran gel chromatography to obtain five components, of which the antioxidant activity of P5 was significantly higher than that of other components(p<0.5). The results showed that the purification effect was good, and P1~P5 all had a single band, which was below 11 kDa.
作者
杨洁茹
刘海波
李晴
郑雪珂
朱静
Yang Jieru;Liu Haibo;Li Qing;Zheng Xueke;Zhu Jing(Xinyang Agriculture and Forestry College,Xinyang 464000)
出处
《粮食与食品工业》
2022年第5期4-9,共6页
Cereal & Food Industry
基金
河南省重点研发与推广专项(科技攻关)项目“大别山油茶粕降脂多肽的分子表征及应用关键技术研究”(212102110314)
信阳农林学院高水平科研孵化器建设基金项目(FCL202014)
河南省高等学校青年骨干教师培养计划(2019GGJS264)
河南省青年科学基金项目(212300410228)。
关键词
油茶饼粕
多肽
分离纯化
抗氧化活性
camellia oleifera cake meal
polypeptide
isolation and purification
antioxidant activity
