Nahlsgen和胶原肽对人皮肤成纤维细胞的保护作用和胶原蛋白合成的影响

Effects of Nahlsgen and collagen hydrolysate on protection and collagen synthesis in human skin fibroblasts

在细胞水平上研究了Nahlsgen和胶原肽对人皮肤成纤维细胞(human skin fibroblast,HSF)的保护作用及胶原蛋白生成能力的影响。通过紫外线中波(ultraviolet B,UVB)照射HSF细胞,建立光损伤细胞模型。分别使用噻唑蓝比色法(methyl thiazolyl tetrazolium,MTT)和二氯二氢荧光素二乙酸酯法(2′,7′-dichlorofluorescin diacetate,DCFH-DA),测定Nahlsgen和胶原肽对光老化HSF的增殖能力以及活性氧(reactive oxygen species,ROS)水平的影响;在Nahlsgen、胶原肽和复配共培养的作用下,测定细胞内产生的羟脯氨酸含量,以及利用实时荧光定量PCR法和蛋白免疫印迹法对HSF的胶原蛋白和弹性蛋白生成能力进行评价。结果表明,Nahlsgen和胶原肽可以促进光老化细胞有效增殖,降低ROS簇含量来保护HSF免受紫外线损伤,也可促进正常HSF胶原蛋白和弹性蛋白的表达,二者复配干预效果更优。研究显示,Nahlsgen和胶原肽联合使用,可提高皮肤抗氧化能力,增强皮肤的弹力,从而延缓皮肤衰老。

The effect of Nahlsgen and protein hydrolysate in human skin fibroblasts(HSF)was studied.In this investigation,HSF were irradiated with ultraviolet B(UVB)ray to establish the cell model of light injury.The methyl thiazolyl tetrazolium test(MTT test)was applied to evaluate the effect of Nahlsgen and collagen hydrolysate on cell viability.Dichloro-dihydro-fluorescein diacetate(DCFH-DA)assay was conducted to reactive oxygen species(ROS)production.The effects of Nahlsgen and collagen hydrolysate on hydroxyproline content and the expression of collagen I and elastin of HSF were measured by qRT-PCR and Western Blot.The results indicated that Nahlsgen and collagen hydrolysate improved the cell viability and decreased the levels of ROS to protect HSF from UVB photodamage.Nahlsgen and collagen hydrolysate promoted the expression of collagen and elastin to delay skin aging.