中药靛玉红衍生物E804对肺癌A549细胞增殖、凋亡和分化的影响及其作用机制

Effects of traditional Chinese medicine indirubin derivative E804 on proliferation, apoptosis and differentiation of lung cancer A549 cells and its mechanism

目的:探讨不同浓度靛玉红衍生物E804对非小细胞肺癌(NSCLC) A549细胞增殖、凋亡和分化的影响,阐明其可能的作用机制。方法:体外培养A549细胞,分为对照组(0μmol·L^(-1)E804)和不同浓度(2.5、5.0和10.0μmol·L^(-1)) E804组。采用MTT法检测各组细胞增殖率,细胞划痕实验检测各组细胞迁移能力,软琼脂克隆实验观察各组细胞分化及克隆形成能力,流式细胞术检测各组细胞凋亡率,Western blotting法检测各组细胞中凋亡相关蛋白和Janus激酶1 (JAK1)/信号转导与转录激因活子3 (STAT3)信号通路相关蛋白表达水平。结果:MTT法,与对照组比较,2.5、 5.0和10.0μmol·L^(-1)E804组细胞增殖率明显降低(P<0.05或P<0.01),并呈时间和浓度依赖性。细胞划痕和软琼脂克隆法,与对照组比较,2.5、5.0和10.0μmol·L^(-1)E804组细胞迁移距离和克隆形成数减少(P<0.05或P<0.01)。流式细胞术检测,与对照组比较,2.5、5.0和10.0μmol·L^(-1)E804组细胞凋亡率明显升高(P<0.05或P<0.01)。Western blotting法检测,与对照组比较,2.5、 5.0和10.0μmol·L^(-1)E804组细胞中B细胞淋巴瘤2 (Bcl-2)、磷酸化JAK1 (p-JAK1)和磷酸化STAT3(p-STAT3)蛋白表达水平降低(P<0.05或P<0.01),Bcl-2相关X蛋白(Bax)、裂解的含半胱氨酸的天冬氨酸蛋白水解酶3 (cleaved caspase-3)和裂解的含半胱氨酸的天冬氨酸蛋白水解酶9 (cleaved caspase-9)蛋白表达水平升高(P<0.05或P<0.01)。结论:E804可抑制A549细胞体外增殖、迁移和分化,并诱导其凋亡,其机制可能与下调JAK1/STAT3信号通路相关蛋白表达有关。

Objective:To investigate the effects of different concentrations of traditional Chinese medicine indirubin derivative E804 on the proliferation,apoptosis and differentiation of non-small cell lung cancer(NSCLC) A549 cells,and to clarify its possible mechanism. Methods:The NSCLC A549 cells were cultured in vitro and divided into control group(0 μ mol·L^(-1)E804) and different concentrations(2. 5,5. 0 and 10. 0 μmol·L^(-1))of E804 groups. MTT assay was used to detect the proliferation rates of cells in various groups;cell scratch assay was used to detect cell migration abilities in various groups;soft agar cloning assay was performed to observe cell differentiation and colony formation abilities in various groups;flow cytometry was used to detect apoptotic rates of cells in various groups;Western blotting method was used to detect the expression levels of apoptosis-related proteins and Janus kinase 1(JAK1)/signal transducer and activator of transcription 3(STAT3) signaling pathway-related proteins in the cells in various groups. Results: The MTT assay results showed that compared with control group,the proliferation rates of A549 cells in 2. 5,5. 0 and 10. 0 μmol·L^(-1)E804 groups were significantly decreased(P<0. 05 or P<0. 01) in a time-and concentration-dependent manner;the cell scratch and soft agar cloning methods results showed that compared with control group,the migration distance and the number of colony formation of the cells in 2. 5,5. 0 and 10. 0 μmol·L^(-1)E804 groups were decreased(P<0. 05 or P<0. 01). The flow cytometry results showed that compared with control group,the apoptotic rates in 2. 5,5. 0 and 10. 0 μmol·L^(-1)E804 groups were increased(P<0. 05 or P<0. 01). The Western blotting method results showed that compared with control group,the expression levels of B-cell lymphoma-2(Bcl-2),phosphorylated JAK1(p-JAK1) and phosphorylated STAT3(p-STAT3) proteins in the cells in 2. 5,5. 0 and 10. 0 μmol·L^(-1)E804 groups were decreased(P<0. 05 or P<0. 01),while the expression levels of Bcl-2 related X protein(Bax),cleaved cysteinyl aspartate specific proteinase-3(cleaved caspase-3) and cleaved cysteinyl aspartate specific proteinase-9(cleaved caspase-9)proteins were increased(P<0. 05 or P<0. 01). Conclusion:E804 can inhibit the proliferation,migration and differentiation of A549 cells in vitro,and induce their apoptosis,and its mechanism may be related to the down-regulation of expressions of JAK1/STAT3 signaling pathway-related proteins.