适用于暗场生化传感系统的微流控芯片的研制与验证

Development and Verification of Microfluidic Chip Suitable for Dark Field Biochemical Sensor System

为了提高暗场检测的效率、获取高质量的传感信息,基于实验设备的物理尺寸以及实验环境,通过COMSOL Multiphysics仿真,并采取键合工艺,研制了一种适用于暗场生化传感系统的微流控芯片。该芯片可在同一暗场视野下观察到四个反应区域,实现对多种样品的并行检测。同时由于该设计的连通性,四个反应区域可以两两进行精确对照,对样本检测结果进行定性定量对比。实验中,在暗场显微成像系统下捕获到微流控芯片通入各反应区域的传感单元纳米粒子,测出散射光谱,验证其可行性。暗场显微成像系统和微流控芯片的结合,实现了自动化、集成化的实时原位并行检测,极大地提高了实验效率。

In order to improve the efficiency of dark field detection and obtain high-quality sensing information,a microfluidic chip suitable for dark field biochemical sensing system is developed based on the physical size of experimental equipment and experimental environment,through COMSOL Multiphysics simulation and bonding process.Four reaction regions in the same field of view can be observed,and the parallel detection of multiple samples is realized.At the same time,due to the connectivity of the design,the four reaction areas can be accurately compared in pairs and the sample test results can be compared qualitively and quantitatively.In the experiment,the nanoparticles were captured in the sensing unit of the microfluidic chip under the dark field microscopic imaging system,and the scattering spectral were measured to verify its feasibility.The combination of dark field microscopic imaging system and microfluidic chip realizes automatic and integrated real-time in-situ parallel detection,which greatly improves the experimental efficiency.