SIRT-1、Beclin-1、LC3在宫颈鳞癌组织中的表达及临床意义

Expression and Clinical Significance of SIRT-1, Beclin-1 and LC3 in Cervical Squamous Cell Carcinoma Tissue

目的 探究沉默信息调节因子家族-1(SIRT-1)及自噬相关蛋白Beclin-1和LC3在宫颈鳞癌组织中的表达及临床意义。方法 选取2014年8月—2015年8月手术后病理科保存的正常宫颈组织、宫颈上皮内瘤变(CIN)1级、CIN 2~3级及宫颈鳞癌组织标本各50例。检测SIRT-1、Beclin-1和LC3在不同宫颈组织中的表达情况。收集宫颈鳞癌患者临床、病理和随访资料,探讨SIRT-1、Beclin-1和LC3表达与患者预后的相关性,分析影响宫颈鳞癌患者预后的因素。结果 正常宫颈组织、CIN 1级组织、CIN 2~3级组织和宫颈鳞癌组织SIRT-1阳性率逐渐增加,而Beclin-1和LC3阳性率逐渐下降(P<0.01)。宫颈鳞癌组织中SIRT-1与Beclin-1、LC3呈显著负相关(r=-0.62、-0.57,P<0.01)。年龄≥50岁、FIGOⅢ~Ⅳ期和SIRT-1阳性是宫颈鳞癌患者预后不良的独立危险因素(P<0.05,P<0.01)。结论 在宫颈鳞癌组织中SIRT-1表达上调,Beclin-1、LC3表达下调,且与宫颈鳞癌恶性程度有关。SIRT-1是宫颈鳞癌患者预后不良的独立危险因素。

Objective To investigate the expression and clinical significance of silent information regulator family-1(SIRT-1),Beclin-1 and LC3 in cervical squamous cell carcinoma.Methods From August 2014 to August 2015,50 samples of normal cervical tissue,cervical intraepithelial neoplasia(CIN)grade 1,CIN grade 2-3 and cervical squamous cell carcinoma tissue preserved by Pathology Department after surgery were selected.The expression of SIRT-1,Beclin-1 and LC3 in different cervical tissues was detected.The clinical,pathological and follow-up data of cervical squamous cell carcinoma patients were collected to explore the correlation between the expression of SIRT-1,Beclin-1 and LC3 and prognosis of patients,and to analyze the influencing factors of prognosis of cervical squamous cell carcinoma patients.Results The positive rates of SIRT-1 in normal cervical tissue,CIN grade 1 tissue,CIN grade 2~3 tissue and cervical squamous cell carcinoma tissue gradually increased,while the positive rates of Beclin-1 and LC3 gradually decreased(P<0.01).SIRT-1 was negatively correlated with Beclin-1 and LC3 in cervical squamous cell carcinoma(r=-0.62,-0.57,P<0.01).Age≥50 years,FIGO stageⅢ-Ⅳand SIRT-1 positive were independent risk factors for poor prognosis of cervical squamous cell carcinoma patients(P<0.05,P<0.01).Conclusion The expression of SIRT-1 is up-regulated,while the expression of Beclin-1 and LC3 is down-regulated in cervical squamous cell carcinoma tissues,which is related to the malignancy of cervical squamous cell carcinoma.SIRT-1 is an independent risk factor for poor prognosis of cervical squamous cell carcinoma patients.