GPR183 在喉鳞癌中的表达及其对喉鳞癌细胞增殖的影响

Expression of GPR183 in laryngeal squamous cell carcinoma and its effect on cell proliferation

目的:探讨G蛋白偶联受体183(G protein-coupled receptor 183,GPR183)在喉鳞状细胞癌(以下简称喉鳞癌)中的表达及其对喉鳞癌细胞增殖的影响。方法:利用免疫组织化学方法检测42例喉鳞癌组织和5例癌旁组织中GPR183的表达,并分析GPR183表达与患者临床病理参数的关系。采用定量聚合酶链式反应(quantitative reverse transcription quantitative polymerase chain reaction,qRT-PCR)检测正常人胚肾细胞HEK-293T和喉鳞癌FD-LSC-1和AMC-HN-8中GPR183表达水平。将GPR183小干扰RNA(small interfering RNA,siRNA)转入喉鳞癌细胞株FD-LSC-1、AMC-HN-8后,采用qRT-PCR检测转染效果,CCK-8实验、细胞克隆形成实验检测细胞增殖、流式细胞术检测细胞周期。并进行体内实验,以评估GPR183在体内对喉鳞癌生长的影响。结果:GPR183表达水平在喉鳞癌中显著上调,喉鳞癌患者组织中GPR183表达水平与临床T分期(P<0.001)、淋巴结转移(P=0.002)相关;下调喉鳞癌细胞中GPR183表达水平,显著抑制喉鳞癌细胞在体外的增殖能力,并诱导细胞周期阻滞于G1期;体内实验结果表明敲降GPR183延缓了喉鳞癌生长。结论:GPR183在喉鳞癌中呈高表达,下调GPR183能够抑制喉鳞状细胞癌的增殖。

Objective:To investigate the expression of G protein-coupled receptor 183 in laryngeal squamous cell carcinoma(LSCC)and its influence on cell proliferation.Methods:The level of GPR183 expression in LSCC samples(n=42)and paracancer tissues samples(n=5)were assessed by immunohistochemistry(IHC),and the relationship between GPR183 expression and clinicopathological parameters of patients were statistically evaluated.The expression level of GPR183 in HEK293T cells and laryngeal squamous cell carcinoma FD-LSC-1 and AMC-HN-8 cells were detected by quantitative reverse transcription-quantitative polymerase chain reaction(RT-qPCR).After transfection of GPR183 small interfering RNA(siRNA)into laryngeal squamous cell carcinoma cell lines(FD-LSC-1,AMC-HN-8).qRT-PCR was used to detect transfection effect,CCK-8 cell proliferation assay and cell clone formation assay were used to detect cell proliferation,and flow cytometry was used to detect cell-cycle progression.Additionally,in vivo experiments were performed to evaluate the effect of GPR183 on the growth of laryngeal squamous cell carcinoma.Results:The expression levels of GPR183 was significantly upregulated in laryngeal squamous cell carcinoma.The expression of GPR183 in laryngeal squamous cell carcinoma was correlated with clinical T stage(P<0.001)and lymph node metastasis(P=0.002).Downregulating GPR183 expression level in LSCC cell lines can significantly inhibit the proliferation of tumor cells,and induce cell cycle arrest at G1 phase.In vivo experiments showed that knockdown GPR183 delayed the growth of laryngeal squamous cell carcinoma.Conclusion:GPR183 was over-expressed in laryngeal squamous cell carcinoma.Down regulation of GPR183 can inhibit the proliferation of laryngeal squamous cell carcinoma.